纳秒脉冲电场诱导B16细胞凋亡的机制研究  

Study on the Apoptosis Mechanism of Murine Melanoma B16 CellsStimulated by Nanosecond Pulse Electric Field

作　　者：饶鑫[1] 陈晓东[1,2] 周俊 刘贻尧[3] Yasir Alfadhl[2] RAO Xin;CHEN Xiao-dong;ZHOU Jun;LIU Yi-yao;ALFADHL Yasir(School of Electronic Science and Engineering,University of Electronic Science and Technology of China,Chengdu 611731;School of Electronic Engineering and Computer Science,Queen Mary University of London,London E14NS;School of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu 610054)

机构地区：[1]电子科技大学电子科学与工程学院,成都611731 [2]玛丽女王大学电子工程与计算机科学学院,伦敦E14NS [3]电子科技大学生命科学与技术学院,成都610054

出　　处：《电子科技大学学报》2020年第6期949-954,共6页Journal of University of Electronic Science and Technology of China

基　　金：NSFA联合基金(U1930127);国家自然科学基金(61827806);中央高校基本科研业务费(ZYGX2019J013)。

摘　　要：纳秒脉冲电场具有诱导癌细胞凋亡、抑制肿瘤生长和诱导抗肿瘤免疫的作用。由于纳秒脉冲诱导治疗涉及的通路过多和常规检测特异性生物标志物技术的局限性,其凋亡机制尚未达成共识且暂时没有高效的研究方法。为了深入研究凋亡机制,该文利用蛋白芯片技术和富集分析技术研究了纳秒脉冲电场诱导小鼠黑色素瘤B16细胞的凋亡机制,并推导了纳秒脉冲诱导治疗的凋亡机制。该研究方法对于有此类靶点数目众多的机制研究具有独特的优势,可为设计未来的纳秒脉冲诱导治疗方案提供参考,优化最佳的脉冲参数或增强/抑制特定的分子以获得最佳治疗效果。Nanosecond pulsed electric field(nsPEF)has been demonstrated to induce cancerous cell apoptosis,inhibit tumor growth and elicit antitumor immunity.Caused by the apoptosis mechanism of nanosecond pulse stimulation(NPS)therapy involving too many targets and the limitation of conventional detecting specific biomarker technology,the apoptosis mechanism of NPS has not been reached consensus and there is no efficient method for researching it.In order to gain further insight into the apoptosis mechanism,the paper has studied the murine melanoma B16 cells stimulated by nsPEF by using antibody array.A hypothesis for the pathway of NPS with three steps for the apoptosis mechanism was introduced.The research method possess the unique advantages in this kind of researches with huge number of targets,the obtained results could be helpful for designing future NPS therapies and aid in targeting the specific molecules with the optimal pulse parameters to obtain best therapeutic effect.

关 键 词：蛋白芯片 细胞凋亡 富集分析 纳秒脉冲电场 

分 类 号：TH79[机械工程—仪器科学与技术] Q6-33[机械工程—精密仪器及机械]