二氧化硅包覆银铜纳米颗粒的结构及其抗菌性能  

Structure and antibacterial properties of silica coated silver-copper nanoparticles

作　　者：姜兴茂 刘奇 郭琳 JIANG Xingmao;LIU Qi;GUO Lin(School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan, Hubei 430000, China)

机构地区：[1]武汉工程大学化工与制药学院,湖北武汉430000

出　　处：《纺织学报》2020年第11期102-108,共7页Journal of Textile Research

基　　金：国家自然科学基金面上项目(21878237);武汉市应用基础前沿项目(2108010401011291)。

摘　　要：为研究双金属纳米颗粒间协同抗菌作用及防止金属纳米颗粒团聚,利用气溶胶一步法制备了“火龙果”型高负载量(50%)二氧化硅包覆银铜双金属纳米颗粒抗菌剂Ag-Cu/SiO2。借助X射线衍射仪、透射电子显微镜、电子能谱仪对Ag-Cu/SiO2的结构进行研究,并测试该材料对金黄色葡萄球菌和大肠杆菌的最低抑菌浓度(MIC)及细菌的时间与杀菌曲线,研究了2种细菌胞内活性氧的生成情况。结果表明:银铜双金属纳米颗粒均匀分散在球型二氧化硅内部,呈现“火龙果”型结构;与Cu/SiO2和Ag/SiO2相比,相同负载量(50%)的Ag-Cu/SiO2有更好的抗菌效果,对2种细菌的MIC值均为2μg/mL,在24 h内可以充分抑制细菌生长;Ag-Cu/SiO2生成活性氧的水平明显高于单金属纳米颗粒,致使细菌死亡,从而说明双金属纳米颗粒具有协同抗菌作用。In order to study the synergistic antibacterial effect between bimetallic nanoparticles and prevent metal nanoparticle agglomeration,a″dragon fruit″type of high-load(50%)silica coated silver-copper bimetallic nanoparticle antibacterial agent(Ag-Cu/SiO2)was prepared using the aerosol one-step method.The structure of Ag-Cu/SiO2 was characterized by X-ray diffraction,transmission electron microscopy,electron spectroscopy,and the minimum inhibitory concentration(MIC)and time-kill curves of 50%Ag-Cu/SiO2 against Staphylococcus aureus and Escherichia coli was also studied.The formation of reactive oxygen species(ROS)in bacterial cells were subsequently investigated.The results show that the silver-copper bimetallic nanoparticles uniformly are dispersed in the spherical silica,presenting a"dragon fruit"structure.The Ag-Cu/SiO2 has better antibacterial properties than Cu/SiO2 and Ag/SiO2 with the same loading(50%).The MIC of Ag-Cu/SiO2 against both bacteria was 2μg/mL and the growth of bacteria was fully inhibited within 24 h.The level of ROS produced by Ag-Cu/SiO2 is significantly higher than that of single metal nanoparticles and it causes the bacteria to die,indicating that the bimetallic nanoparticles have synergistic antibacterial effect.

关 键 词：二氧化硅 气溶胶法 抗菌剂 金属纳米颗粒 活性氧 抗菌性能 

分 类 号：O614.12[理学—无机化学]