几种习用代茶植物体外降糖活性研究  

Study on Hypoglycemic Activities of Conventional Substituting-for-Tea Plants in Vitro

作　　者：杨立昌[1,2] 冯淳 杨臣菊 杨菡 廉瑞婷 余正文 YANG Lichang;FENG Chun;YANG Chenju;YANG Han;LIAN Ruiting;YU Zhengwen(School of Life Sciences,Guizhou Normal University,Guiyang 550025,China;Institute of Higher Education,Guizhou Provincial Educational Sciences Academy,Guiyang 550081,China)

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550025 [2]贵州省教育科学院高等教育研究所,贵州贵阳550081

出　　处：《食品科技》2020年第9期62-66,共5页Food Science and Technology

基　　金：国家自然科学基金项目(U1812401,31460068);贵州省教育厅自然科学研究项目(黔教合KY字[2015]335);贵州省微生物-植物互作及代谢调控科技创新人才团队项目(黔科合平台人才[2019]5617号)。

摘　　要：研究藤茶、石崖茶、多穗石柯茶、大叶苦丁茶、小叶苦丁茶、青城山苦丁茶、广西甜茶、老鹰茶、金银花茶、青钱柳茶的体外降糖活性。通过测定其水提物中总黄酮、总多酚含量,以及对各部分的α-葡萄糖苷酶抑制率及DPPH自由基清除率进行比较研究。结果表明:在酸性提取物方面,提取率最高为藤茶((35.76±2.18)%),总黄酮和总多酚含量最高的分别是藤茶((7.83±0.76)%)和大叶苦丁茶((8.31±0.43)%)。10种代茶植物水提物的酸性部位α-葡萄糖苷酶抑制活性较好的为石崖茶(IC50=(0.004±0.001)mg/mL)、老鹰茶(IC50=(0.014±0.001)mg/mL)、藤茶(IC50=(0.022±0.001)mg/mL);DPPH自由基清除活性较好的也为老鹰茶(IC50=(0.009±0.004)mg/mL)、藤茶(IC50=(0.015±0.001)mg/mL)、石崖茶(IC50=(0.015±0.001)mg/mL)。藤茶、石崖茶和老鹰茶体外降糖活性相对较好,可考虑用于糖尿病患者的辅助治疗。The hypoglycemic activities of ten conventional substituting-for-tea in vitro were studied systematically.Total flavonoids and total polyphenols in water extracts of rattan tea,Shi-ya tea,Lithocarpus Polyst achys Rehd tea,Ilex latifolia Thumb,Ilex Kudingcha,Qing-cheng-shan Ku-ding tea,Rubus Suavissimus S.Lee,Litsea coreana,Honeysuckle tea and Cyclocarya Paliurus tea were determined.As well as theα-glucosidase inhibition rate and DPPH free radical scavenging rate of each part were compared.For the acid extracts from substituting-for-tea,the highest extraction rate was rattan tea((35.76±2.18)%),the highest content of total flavonoids and polyphenols was rattan tea((7.83±0.76)%)and Kuding tea((8.31±0.43)%),respectively.The betterα-glucosidase inhibition rates were Shi-ya tea(IC50=(0.004±0.001)mg/mL),Litsea coreana(IC50=(0.014±0.001)mg/mL)and rattan tea(IC50=(0.022±0.001)mg/mL).However,the better DPPH free radical scavenging rate were Litsea coreana(IC50=(0.009±0.004)mg/mL),rat tan tea(IC50=(0.015±0.001)mg/mL)and Shi-ya tea(IC50=(0.015±0.001)mg/mL).Rattan tea,Shi-ya tea and Litsea coreana can be considered as an adjuvant treatment of diabetic patients for relatively better hypoglycemic activity in vitro.

关 键 词：代茶植物 酸性提取物 体外降糖活性 

分 类 号：R284.2[医药卫生—中药学]