基于公共数据库挖掘的ACE2肺部表达差异及与新型冠状病毒肺炎的相关性的功能预测  

Functional prediction of ACE2 lung expression differences and correlation with COVID-19 pneumonia based on public database mining

作　　者：荣健 王文欣[1] 颉迎新 周笳 王宣 RONG Jian;WANG Wen-xin;JIE Ying-xin;ZHOU Jia;WANG Xuan(Tianjin Hospital,Tianjin300210,China)

机构地区：[1]天津医院急诊内科,天津300210

出　　处：《中华医院感染学杂志》2020年第19期2881-2885,共5页Chinese Journal of Nosocomiology

基　　金：天津市科研计划基金资助项目(2792839)。

摘　　要：目的金属肽酶中的血管紧张素转换酶2(Angiotensin-converting enzyme 2,ACE2)被确定为新型冠状病毒的功能性受体。本研究旨在探究ACE2在新型冠状病毒引起肺炎(以下简称"新冠肺炎")中的表达差异。方法对GSE42834数据集获得的112个新冠肺炎患者的新冠肺炎组织和18个肺炎患者的正常肺组织的测序结果进行差异分析,设定差异分析的条件是logFC绝对值>1,P<0.05。使用人蛋白质数据库进一步验证ACE2在正常肺组织的表达情况。Coexpedia数据库进行ACE2共表达基因的检索。Metascape对共表达基因进行GO和KEGG富集分析,以便进行预测ACE2在机体发挥的功能以及可能参与的信号通路。结果GSE42834数据库获得具有差异性表达的基因共有249个,其中上调的基因有156个,下调的基因有93个。上调变化较大的基因有VNN1,ACE2,OLFM4,CD67,CD64,下调变化较大的基因有GNLY,FGFBP2,CLIC3,D4S234E和KLRB1。差异结果分析可见ACE2属于上调基因。组间表达结果提示与正常肺组织相比,ACE2在新冠肺炎组织中的确表达上调,人蛋白质数据库免疫组化结果提示,ACE2在正常肺组织表达阴性。GO和KEGG富集分析和可视化发现ACE2参与介导I型干扰素信号通路和Toll样受体信号通路。结论ACE2在新冠肺炎患者中可能高表达,有望成为治疗新冠肺炎的新靶点。OBJECTIVE Angiotensin-converting enzyme 2(ACE2)in metallopeptidase has been identified as a functional receptor for COVID-19.The purpose of this study was to explore the difference in the expression of ACE2 in COVID-19-induced pneumonia(hereinafter referred to as"COVID-19").METHODS The difference analysis of sequencing results of 112 new coronary pneumonia patients and normal lung tissue of 18 pneumonia patients obtained by GSE42834 data set was performed,and the condition of the difference analysis were set as the absolute value of LogFC>1,P<0.05.The human protein database was used to further verify the expression of ACE2 in normal lung tissue.Coexpedia database was used to search for ACE2 coexpression genes.Metascape was used to enrich the co-expressed genes with GO and KEGG analysis,so as to predict the function of ACE2 in the boy and the possible signal pathways involved.RESULTS A total of 249 differentially expressed genes were obtained from GSE42834 database,of which 156 genes were up-regulated and 93 genes were down-regulated.The major up-regulated genes were VNN1,ACE2,OLFM4,CD67,CD64,and the major down-regulated genes were GNLY,FGFBP2,CLIC3,D4 S234 E and KLRB1.Analysis of the difference results showed that ACE2 was an up-regulated gene.The results of intergroup expression suggested that ACE2 was indeed up-regulated in new coronary pneumonia tissue compared with normal lung tissue.The results of immunohistochemistry of the human protein databases suggested that ACE2 was negatively expressed in normal lung tissue.The enrichment analysis and visualization of GO and KEGG found that ACE2 was involved in mediating type I interferon signal pathway and Toll-like receptor signal pathway.CONCLUSION ACE2 may be highly expressed in patients with new coronavirus pneumonia,and it is expected to become a new target for the treatment of new coronavirus pneumonia.

关 键 词：金属肽酶中的血管紧张素转换酶2 新型冠状病毒肺炎 信号通路 基因表达 

分 类 号：R563.1[医药卫生—呼吸系统]