CD14启动子-159C/T多态性与呼吸道肺炎链球菌感染的相关性  

Correlation between polymorphism of CD14 promoter-159C/T and respiratory Streptococcus pneumoniae infection

作　　者：周捷 刘海潮 夏飞 袁静 ZHOU Jie;LIU Hai-chao;XIA Fei;YUAN Jing(Wuhan University of Technology Hospital,Wuhan,Hubei 430070,China;不详)

机构地区：[1]武汉理工大学医院内科,湖北武汉430070 [2]中国人民解放军中部战区总医院,湖北武汉430060

出　　处：《中华医院感染学杂志》2020年第19期2931-2935,共5页Chinese Journal of Nosocomiology

基　　金：湖北省自然科学基金资助项目(712668)。

摘　　要：目的探究CD14启动子-159C/T多态性与呼吸道肺炎链球菌感染的相关性。方法选取2016年7月-2018年6月于武汉理工大学医院就诊的呼吸道肺炎链球菌感染患者87例作为试验组,并选取同期来医院体检的健康志愿者80例作为对照组。采用Taqman探针荧光定量PCR法对CD14启动子-159C/T进行基因分型分析,观察-159C/T位点的基因型及等位基因频率在两组间的分布特征,分析多态位点与呼吸道肺炎链球菌感染的相关性。结果试验组携带显性等位基因纯合型CC的频率31.03%高于对照组的15.00%,隐性等位基因纯合型TT的频率39.08%低于对照组的56.25%;试验组中C等位基因频率为45.98%高于对照组,T等位基因的频率为54.02%,低于试验组;-159C/T等位基因频率与呼吸道肺炎链球菌感染之间存在一定的相关性(r=0.171,P=0.002)。结论CD14启动子-159C/T多态性可能与呼吸道肺炎链球菌感染相关,位点中C等位基因可能是风险等位基因。OBJECTIVE To explore the relationship between CD14 promoter-159 C/T polymorphism and respiratory tract Streptococcus pneumoniae infection.METHODS A total of 87 patients with respiratory tract Streptococcus pneumoniae infection who were treated in Wuhan University of technology hospital from Jul.2016 to Jun.2018 were selected as the experimental group,and another 80 healthy volunteers who underwent physical examination during the same period were enrolled as control group.The genotyping of CD14 promoter-159 C/T was analyzed by Taqman probe fluorescence quantitative PCR.The distribution features of genotype and allele frequency at-159 C/T locus in both groups were observed.The correlation between polymorphic sites and respiratory Streptococcus pneumoniae infection was analyzed.RESULTS The frequency of homozygous CC with dominant allele in experimental group was 31.03%,higher than that in control group(15.00%);while the frequency of TT with recessive allele homozygous was 39.08%,lower than that in control group(56.25%).The frequency of C allele in experimental group was 45.98%,higher than that in control group(29.38%),and frequency of T allele was 54.02%,lower than that in control group(70.63%).There was a certain correlation between the frequency of-159 C/T allele and respiratory Streptococcus pneumoniae infection(r=0.171,P=0.002).CONCLUSION The polymorphism of CD14 promoter-159 C/T might be related to respiratory Streptococcus pneumoniae infection,and the C allele in the locus might be the risk allele.

关 键 词：白细胞分化抗原14 肺炎链球菌感染 多态性 基因 

分 类 号：R563.1[医药卫生—呼吸系统]