Meta-Analysis of Polymerase Chain Reaction in the Detection of Human Leptospirosis  

作　　者：MA Lina MA Ya LUO Bailing MA Bishu YAN Jie LIN Xu'ai 

机构地区：[1]School of Medicine,Zhejiang University,Hangzhou 310058,Zhejiang,China [2]Department of Medical Teaching in Dingxi Campus,Gansu University of Chinese Medicine,Dingxi 743000,Gansu,China [3]Department of Petroleum Chemical Engineering,Lanzhou Petrochemical College of Vocational Technology,Lanzhou 730060,Gansu,China [4]Department of Medical Microbiology and Immunology,Haiyuan School of Kunming Medical University,Kunming 650106,Yunnan,China

出　　处：《Wuhan University Journal of Natural Sciences》2020年第5期387-394,共8页武汉大学学报（自然科学英文版）

基　　金：the National Natural Science Foundation of China(81271781);the Natural Science Foundation of Zhejiang Province(LY17H190002);the Higher Education Institutions of Gansu Province of China(2019B-230)。

摘　　要：Leptospirosis,a major zoonotic disease widespread in the world,would infect humans and other animals by direct contacting with the soil or water contaminated by the pathogenic leptospires.This report analyzes the accuracy of polymerase chain reaction(PCR)assay used in the detection of leptospira specific genes in humans.Seventeen published articles which included 2526 samples satisfied all inclusion standards and were applied to meta-analysis.Pooled sensitivity and specificity were 0.73(95%CI 0.70-0.76)and 0.94(95%CI 0.93-0.95),respectively.Heterogeneity was statistically significant among studies,but none of the sources for heterogeneity(disease stage,PCR type,targeted genes)could adequately interpret this finding.Meta-regression advised that real-time PCR(qPCR)targeted 16 s rRNA would be the best choice for early detection of cases during the acute stage of the disease and was accounted as a good screening tool in all stages of the disease.

关 键 词：human leptospirosis polymerase chain reaction META-ANALYSIS sensitivity SPECIFICITY 

分 类 号：R445[医药卫生—影像医学与核医学]