Ⅰ群禽腺病毒FAdV-4和FAdV-8灭活疫苗的制备及免疫效果  

Development and efficacy of bivalent inactivated oil-emulsion vaccine for FAdV-4 and FAdV-8 of fowl adenovirus group-Ⅰ

作　　者：张林吉[1] 迟兰[1] 李心海[1] 王玉燕[1] 任士飞[1] 张小荣[2] 吴艳涛[2] ZHANG Lin-ji;CHI Lan;LI Xin-hai;WANG Yu-yan;REN Shi-fei;ZHANG Xiao-rong;WU Yan-tao(Xuzhou Vocational College of Bioengineering,Xuzhou,Jiangsu 221006,China;College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China)

机构地区：[1]徐州生物工程职业技术学院,江苏徐州221006 [2]扬州大学兽医学院,江苏扬州225009

出　　处：《中国兽医学报》2020年第9期1715-1719,共5页Chinese Journal of Veterinary Science

基　　金：徐州市科技局农业科技农业高技术攻关资助项目(KC16NG062)。

摘　　要：为提高鸡心包积液-肝炎综合征(hydropericardium hepatitis syndrome,HHS)和鸡包涵体肝炎(inclusion body hepatistis,IBH)疾病的防控能力,研制有效防控Ⅰ群禽腺病毒(FAdV)疫苗。本研究将实验室分离鉴定的FAdV-4 SN2016株和FAdV-8标准毒株,采用鸡胚增殖方式,优化最佳接种剂量、收获时间及甲醛的灭活条件,病毒抗原以3∶1比例混合,制备双价油乳剂灭活疫苗,检验疫苗性状、监测免疫后雏鸡的抗体水平及对其免疫保护效果。病毒采用卵黄囊接种,尿囊液中病毒粒子含量分别达到4.6×10^8和7.8×10^7拷贝/mL;灭活条件为终浓度1.5‰的甲醛灭活24 h;制备的疫苗为油包水剂型,物理性状稳定,无菌检测合格,4℃保存期可达12个月;雏鸡免疫抗体水平监测结果显示,免疫后3~4周抗体水平达到高峰,第6周依然维持较高水平,制备的双价油乳剂灭活疫苗可以有效抵抗单病毒和双病毒的攻击。研究结果显示制备的FAdV-4 SN2016和FAdV-8双价油乳剂灭活疫苗性状稳定、保存期长,免疫效果好,能有效抵抗病毒攻击,为HHS和IBH疫病的防控提供有效手段。The experiment was conducted to development vaccine of fowl adenovirus group-Ⅰ,in order to improve the prevention ability of hydropericardium hepatitis syndrome(HHS)and of inclusion body hepatistis(IBH).In this research,the FAdV-4 SN2016 strain previously isolated by our laboratory and FAdV-8 standard strain are proliferated by chicken embryo,the best inoculation dose,harvest time and formaldehyde inactivation conditions were optimized.Mixing virus antigens as the ratio of 3∶1,producted the bivalent oil-emulsion inactivated vaccine.The vaccine characters were investigated,immune antibody level was monitored and immunoprotective effect test was tested.Inoculated in yolk sac,the virion content reached 4.6×10^8 and 7.8×10^7 copies/mL respectively.The viruses were inactivated by formaldehyde with a final concentration of 1.5‰lasting 24 h.The vaccine showed white oil-emulsion and could be preserved 12 months at 4℃.The antibody level reached the OD450 value peak between week 3 and week 4 after immunization and remained higher even after 6 weeks.The vaccine showed 10^0%protection rate,and could effectively resist the attacks of monovirus and divirus.All the results proved that the FAdV-4 SN2016 and FAdV-8 bivalent oil-emulsion inactivated vaccine has long protection duration,good immune effects,and can very effectively resist infection of the corresponding virus.

关 键 词：HHS IBH Fadv-4 SN2016 FAV-8 双价油乳剂灭活疫苗 

分 类 号：S852.65[农业科学—基础兽医学]