人工感染禽腺病毒血清4型病毒对鹅组织中免疫相关因子基因表达的影响  

Effects of artificially infected fowl adenovirus serotype 4 on the expression of immune related factors in goose tissues

作　　者：任梦婷 姜磊 刘亮亮[1,2] 李慧昕 张莉莉[1,2] 侯雨彤 马得莹 REN Meng-ting;JIANG Lei;LIU liang-liang;LI Hui-xin;ZHANG Li-li;HOU Yu-tong;MA De-ying(College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China;National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China)

机构地区：[1]东北农业大学动物科学技术学院,黑龙江哈尔滨150030 [2]中国农业科学院,哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江哈尔滨150069

出　　处：《中国兽医学报》2020年第9期1726-1736,共11页Chinese Journal of Veterinary Science

基　　金：黑龙省现代农业产业技术协同创新推广体系,国家重点研究发展计划资助项目(2017YFD0500105);国家“十二五”科技支撑计划资助项目(2015BAD12B03)。

摘　　要：以鹅为研究对象,人工感染禽腺病毒(fowl adenovirus,FAdV),分别在攻毒后36,72 h和3,7 d,采集脾脏、哈德氏腺、骨髓、法氏囊和盲肠扁桃体,运用实时荧光定量PCR,检测鹅感染FAdV-4后,免疫器官中FAdV-4载量及免疫相关因子基因表达差异,初探FAdV-4对鹅致病性以及鹅对该病毒的天然免疫反应机制。结果表明,与对照组相比,在法氏囊、盲肠扁桃体和哈德氏腺中检测到FAdV-4,感染初期TLR2和TLR3显著上调,感染后期TLR5和TLR15显著下调;AvBD1、AvBD9、AvBD10和AvBD16在部分组织中表达量显著升高,且大部分细胞因子表达受到抑制。研究表明,感染FAdV-4后,会激活宿主先天免疫反应,为研究FAdV-4与宿主先天免疫的相互作用提供新的思路。In this experiment the spleen,Harderian gland,bone marrow,bursa of Fabricius and cecal tonsil were collected at 36,72 h,3 and 7 d post infection(hpi or dpi).When the goose was infected with FAdV-4,differential expression of FAdV-4 load and immune-related factors in immune organs were detected by RT-qPCR.And the pathogenicity of FAdV-4 to goose and the natural immune response mechanism of goose to the virus were investigated.The results showed that FAdV-4 was detected in the bursa of Fabricius,cecal tonsil and Harderian glands,and TLR2 and TLR3 were significantly up-regulated at the early stage of infection,and TLR5 and TLR15 were significantly down-regulated at the late stage of infection;the expression levels of AvBD1,AvBD9,AvBD10 and AvBD16 were significantly increased in some tissues;and most of the cytokine expression were inhibited compared with the control group.The results showed that the host innate immune response was activated after infection with FAdV-4,The present funding provided new insights into the interaction between FAdV-4 and host innate immunity.

关 键 词：禽β-防御素 TOLL样受体 天然免疫 禽腺病毒 

分 类 号：S852.4[农业科学—基础兽医学]