四川石渠县牦牛体表蜱携带无形体和疏螺旋体的分子检测  

Molecular identification of Anaplasma species and Borrelia species in ticks collected from yaks in Shiqu of Sichuan Province

作　　者：唐天才 刘城成 魏巍[2] 袁东波[3] 郭莉[3] 侯巍[3] 莫茜[3] 阳爱国[3] 杨晓农[1] 郝力力[1] TANG Tian-cai;LIU Cheng-cheng;WEI Wei;YUAN Dong-bo;GUO Li;HOU Wei;MO Xi;YANG Ai-guo;YANG Xiao-nong;HAO Li-li(College of Life Science and Technology,Southwest Minzu University,Chengdu 610041,China;China Animal Disease Prevention and Control Center,Beijing 100125,China;Center for Animal Disease Control and Prevention in Sichuan Province,Chengdu 610041,China)

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]中国动物疫病预防控制中心,北京100125 [3]四川省动物疫病预防控制中心,四川成都610041

出　　处：《中国兽医学报》2020年第9期1758-1764,共7页Chinese Journal of Veterinary Science

基　　金：四川省教改资助项目“兽医专业学位研究生教育实践基地建设”。

摘　　要：为了解四川省石渠县牦牛体表寄生蜱携带无形体与疏螺旋体的种类和感染情况。采集牦牛体表寄生蜱,经形态学和分子生物学鉴定,确定蜱种类。再采用巢式PCR分别扩增无形体科16S rRNA基因和疏螺旋体属的16S rRNA与flaB基因片段,并对阳性产物进行测序、比对和系统进化树构建,从而确定无形体和疏螺旋体的种类。结果显示,共采集到818只蜱,其中西藏革蜱占78.97%(646/818)、青海血蜱占21.03%(172/818)。所有蜱中有132只检出无形体,阳性率为16.14%;8只检出疏螺旋体,阳性率为0.98%;3只蜱混合感染牛无形体和疏螺旋体。经进一步分析,仅在青海血蜱中检测到牛无形体(Anaplasma bovis)、埃立克体(Ehrlichia)和疏螺旋体(Borrelia),其阳性率分别为6.98%(12/172),2.33%(4/172)和4.65%(8/172);仅在西藏革蜱中检测到绵羊无形体(Anaplasma ovis),其阳性率为17.96%(116/646)。此外,获得的疏螺旋体16S rRNA和flaB基因序列均与分离自日本大刺血蜱中的Borrelia sp.HM菌株亲缘关系最近。结果提示,四川石渠县存在牛无形体、绵羊无形体、埃立克体未定种以及疏螺旋体未定种,存在感染人的风险。In order to investigate infection of Anaplasma spp and Borrelia spp of ticks in Shiqu county of Sichuan Province.The ticks collected from yaks were classified by morphological and molecular identification.The partial sequences of 16 SrRNA gene of Anaplasma spp,16 S rRNA and flaB gene of Borrelia spp were amplified by nested PCR,respectively.The positive products were sequenced and compared through the NCBI database.Phylogenetic trees were constructed based on 16 S rRNA and flaB gene to determine the species of Anaplasma and Borrelia.The results showed that a total of 818 ticks were collected,The most abundant species was Dermacentor everestianus(78.97,646/818)followed by Haemphysalis qinghaiensis(21.03%,172/818).In ticks collected from yak,Anaplasma spp DNA was detected with a positive rate of 16.14%(132/818)and 8 ticks were found to be Borrelia spp positive,with a detection rate of 0.98%.Mixed infection of Anaplasma bovis and Borrelia sp was observed in 3 ticks.Further analysis showed that Anaplasma bovis,Ehrlichia sp and Borrelia sp were only detected in Haemphysalis qinghaiensis,with a positive rates of 6.98%(12/172),2.33%(4/172)and 4.65%(8/172),respectively;Anaplasma ovis was detected only in Dermacentor everestianus,with a positive rate of 17.96%(116/646).In addition,phylogenetic analysis based on 16 SrRNA and flaB showed Borrelia sp Shiqu was most closely related to Borrelia sp.HM strain isolated in Haemphysalis megaspinosa from Japan.In this study,results showed that there are Anaplasma bovis,Anaplasma ovis,Ehrlichia sp and Borrelia spi.n Shiqu County,suggesting there exsits a high risk of human infection.

关 键 词：蜱 无形体 疏螺旋体 分子检测 四川石渠县 

分 类 号：S852.63[农业科学—基础兽医学]