河北地区猪源克雷伯菌Ⅰ型整合子-基因盒的捕获与耐药基因携带情况及其致病性  

Correlation between antimicrobial resistance and class Ⅰ integron-gene cassette of Klebsiella isolated from swine of Hebei Province and its pathogenicity

作　　者：王梦佳 孟宪华 宣国彦 刘莹[1] 韩磊 彭志豪 范京惠[1] 左玉柱[1] 李清艳[1] WANG Meng-jia;MENG Xian-hua;XUAN Guo-yan;LIU Ying;HAN Lei;PENG Zhi-hao;FAN Jing-hui;ZUO Yu-zhu;LI Qing-yan(College of Veterinary Medicine/Agriculture Science and Education Center,Hebei Agricultural University,Baoding,Hebei 071001,China;Hebei Provincial Animal Husbandry Station,Shijiazhuang 050035,China;Xinhua District Agricultural Burean of Cangzhou City,Cangzhou,Hebei 061000,China)

机构地区：[1]河北农业大学动物医学院/科教兴农中心,河北保定071001 [2]河北省畜牧总站,河北石家庄050035 [3]沧州市新华区农业农村局,河北沧州061000

出　　处：《中国兽医学报》2020年第9期1765-1769,1776,共6页Chinese Journal of Veterinary Science

基　　金：河北省重点研发计划资助项目(19226622D);河北省农业产业技术体系生猪创新团队资助项目(HBCT2018110207)。

摘　　要：为探究Ⅰ型整合子-耐药基因盒对克雷伯菌耐药性产生和传播的影响及该菌的致病性,针对本实验室分离到的猪源克雷伯菌进行Ⅰ型整合子扩增及可变区序列分析,分析其结构及与耐药性关系,建立分离菌株感染动物模型,从临床症状、病理变化、细菌学检查和组织病理学4个方面开展研究。结果显示,该菌株主要以介导甲氧苄啶类耐药drfA为主,与其药敏试验耐药表型一致,感染动物模型发生实质器官病变且在病理切片中可见组织中大量炎性细胞浸润,肝、脾血窦开放和充血等病变。To understand the drug resistance and the spread of integron-gene cassette of Klebsiella,type I integron amplification and variable region sequence analysis of Klebsiella Suis isolated from Hebei province were performed to analyze its structure and its relationship with drug resistance,and establish the animal model of infection by the isolated strain.The study was conducted from clinical symptoms,pathological changes,bacteriological tests and histopathology.The result showed that the strain is mainly composed of drfA,which mediates drug resistance of trimethopenzidine,and it is consistent with the drug resistance phenotype of drug sensitivity test.Parenchymal organ lesions occurred in the infected animal model,and a large number of inflammatory cell infiltration could be seen in the tissues in the pathological sections,as well as liver and spleen blood sinus opening and congestion.

关 键 词：克雷伯菌 整合子 耐药基因 致病性 动物模型 

分 类 号：S852.61[农业科学—基础兽医学] R535[农业科学—兽医学]