β-catenin在lα,25-(OH)2D3调控小鼠体外破骨细胞形成中的作用  

Role of β-catenin in mice osteoclastogenesis controlled by lα,25-(OH)2D3 in vitro

作　　者：周佳桦 张雨蓓 张松彬 张闯 闵雯嫣 赵鸿雁[1] 刘宗平[1] 顾建红[1] ZHOU Jia-hua;ZHANG Yu-bei;ZHANG Song-bin;ZHANG Chuang;MIN Wen-yan;ZHAO Hong-yan;LIU Zong-ping;GU Jian-hong(Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses/Jiangsu Key Laboratory of Zoonosis,College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China)

机构地区：[1]扬州大学兽医学院,江苏高校动物重要疫病与人兽共患病防控协同创新中心/江苏省人兽共患病学重点实验室,江苏扬州225009

出　　处：《中国兽医学报》2020年第9期1810-1816,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31872534,31872533,31702304);江苏省自然科学基金资助项目(BK20181452);江苏高校优势学科建设工程资助项目(PAPD);扬州大学大学生创新创业训练计划项目/学术科技创新基金项目(x20180609)。

摘　　要：旨在探究β-catenin在lα,25-(OH)2D3调控小鼠体外破骨细胞(osteoclast,OC)形成中的作用。体外诱导小鼠OC形成,添加1α,25-(OH)2D3观察其对OC形成及β-连环蛋白(β-catenin)表达的影响;敲低β-catenin进一步观察1α,25-(OH)2D3对OC形成的影响。结果显示,1α,25-(OH)2D3极显著(P<0.01)抑制体外培养小鼠OC形成和骨吸收活性。敲低β-catenin,OC数量及OC形成标志物基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)蛋白和激活T-细胞核因子1(nuclear factor of activated T cells,cytoplasmic 1,Nfatc1)mRNA表达显著(P<0.05)升高。1α,25-(OH)2D3可促进OC形成过程中β-catenin基因Ctnnb1 mRNA的表达,但敲低β-catenin基因,1α,25-(OH)2D3仍可抑制OC形成。试验表明,β-catenin可抑制体外培养OC形成,但对1α,25-(OH)2D3抑制OC形成无影响。To investigate the role ofβ-catenin in mice osteoclastogenesis controlled by lα,25-(OH)2D3 in vitro,mice osteoclasts(OC)were induced in vitro and treated by 1α,25-(OH)2D3.The number of OC and the expression ofβ-catenin were detected before and afterβ-catenin knocking-down.The results showed that,1α,25-(OH)2D3 significantly inhibited mice OC formation and bone resorption activity in vitro.OC number,the expression of matrix metalloproteinase-9(MMP-9)protein and nuclear factor of activated T cells,cytoplasmic 1(Nfatc1)mRNA were upregulated afterβ-catenin knocking-down.During OC formation,1α,25-(OH)2D3 upregulated the expression ofβ-catenin gene Ctnnbl mRNA.However,when knocking downβ-catenin gene,1α,25-(OH)2D3 can still significantly inhibit OC formation.In conclusion,β-catenin inhibited mice OC formation in vitro,but had no effect on OC formation inhibited by 1α,25-(OH)2D3.

关 键 词：Β-连环蛋白 1α 25-(OH)2D3 小鼠 破骨细胞 骨 

分 类 号：S852.2[农业科学—基础兽医学]