Prohibitin(PHB)interacts with AKT in mitochondria to coordinately modulate sperm motility  

作　　者：Xiao-Hui Li Ran-Ran Chai Guo-Wu Chen Ling-Fei Zhang Wen-Jing Tan-Tai Hui-Juan Shi Patricia A Martin-DeLeon Wai-Sum O Hong Chen 

机构地区：[1]Department of Anatomy,Histology and Embryology,Key Laboratory of Medical Imaging Computing and Computer Assisted Intervention of Shanghai,School of Basic Medical Sciences,Shanghai Medical College,Fudan University,Shanghai 200032,China [2]Shanghai Ji'Ai Genetics and IVF Institute,Obstetrics and Gynecology Hospital,Institute of Reproduction and Development,Fudan University,Shanghai 200011,China [3]Key Lab of Reproduction Regulation of NPFPC-Shanghai Institute of Planned Parenthood Research,Fudan University Reproduction and Development Institution,Shanghai 200032,China [4]Department of Biological Sciences,University of Delaware,Newark,DE 19716-2590,USA [5]School of Biomedical Sciences,The University of Hong Kong,Hong Kong SAR,China

出　　处：《Asian Journal of Andrology》2020年第6期583-589,共7页亚洲男性学杂志（英文版）

基　　金：This project was funded by grants from the National Natural Science Foundation of China(No.81270738)and the Major State Basic Research Development Program of China(No.2014CB943104).

摘　　要：Prohibitin(PHB),an evoluti on arily con served mitochondrial inner membra ne protein,is highly expressed in cells that require strong mitoch on drial function.Recently,we dem on strated that the deleti on of Phb in spermatocytes results in impaired mitochondrial function.In addition,PHB expression in the mitochondrial sheath of human sperm has a significantly negative correlation with mitochondrial reactive oxygen species levels,but a positive one with mitochondrial membrane potential and sperm motility.These results suggest that mitochondrial PHB expression plays a role in sperm motility.However,the mechanism of PHB-mediated regulation of sperm motility remai ns unk nown.Here,we dem on strate for the first time that PHB interacts with protei n kinase B(AKT)and exists in a complex with phospho-PHB(pT258)and phospho-AKT in the mitochondrial sheath of murine sperm,as determined using colocalization and coimmunoprecipitation assays.After blocking AKT activity using wortmannin(a phosphatidylinositol 3-kinase[PI3K]inhibitor),murine sperm have significantly(P<0.05)decreased levels of phospho-PHB(pT258)and the total and progressive motility.Furthermore,significantly(P<0.05)lower levels of phospho-PI3K P85 subunit a+γ(pY199 and pY46)and phospho-AKT(pS473;pT308)are found in sperm from infertile asthenospermic and oligoasthenospermic men compared with no rmospermic subjects,which suggest a reduced activity of the PI3K/AKT pathway in these in fertile subjects.Importantly,these sperm from infertile subjects also have a significantly(P<0.05)lower level of phospho-PHB(pT258).Collectively,our findings suggest that the interaction of PHB with AKT in the mitochondrial sheath is critical for sperm motility,where PHB phosphorylation(pT258)level and PI3K/AKT activity are key regulatory factors.

关 键 词：male in fertility prohibitin(PHB) protein kinase B(AKT) sperm motility 

分 类 号：R697[医药卫生—泌尿科学]