Comparison of ART outcomes in men with altered mRNA protamine 1/protamine 2 ratio undergoing intracytoplasmic sperm injection with ejaculated and testicular spermatozoa  

作　　者：Jonas Sarasa Maria Enciso Laura Garcia Andrea Leza Klaus Steger Jon Aizpurua 

机构地区：[1]iGLS,Alicante 03540,Spain,Molecular Andrology,Justus-Liebig-UDiversity Giessen,Giessen 35385,Germany [2]IVF-Spain,Alicante 03540,Spain,Molecular Andrology,Justus-Liebig-UDiversity Giessen,Giessen 35385,Germany [3]Department of Urology,Pediatric Urology and Andrology,Molecular Andrology,Justus-Liebig-UDiversity Giessen,Giessen 35385,Germany

出　　处：《Asian Journal of Andrology》2020年第6期623-628,共6页亚洲男性学杂志（英文版）

基　　金：The authors thank all the patients for consenting to participate in this study.Furthermore,the technical assistance of Barbara Frohlich and Mareike Buch-Heberling is gratefully acknowledged.KS was supported by a Research Grant from the University Medical Center Giessen and Marburg(UKGM,project 29/2015GI).This research did not receive any other specific grant from funding agencies in the public,commercial,or not-for-profit sectors.

摘　　要：Assisted reproductive technologies invoIving the use of spermatozoa and eggs for in vitro fertilization(IVF)have come as the solution for many infertile couples to become parents.However,in some cases,the use of ejaculated spermatozoa delivers poor IVF performance.Some studies have suggested the use of testicular spermatozoa in severe male in fertility cases,but no guideli nes regarding their utilization are currently available.In the present study,we found the mRNA protamine 1/protamine 2(P1/P2)ratio to be a valuable biomarker of poor sperm function that could be used as a diagnostic key for the identification of cases that would benefit from the use of testicular spermatozoa.A total of 23 couples undergoing egg donation cycles with at least one previous cycle failure were studied.All couples underwent two consecutive intracytoplasmic sperm injection(ICSI)cycles with either ejaculated or testicular spermatozoa(TESA).The sperm mRNA P1/P2 ratio,fertilization rate,blastocyst rate,and pregnancy and live birth rate were compared.Results showed improved ICSI and clinical outcomes in cycles with testicular spermatozoa in men with altered mRNA P1/P2 ratios.TESA cycles presented significantly higher rates of fertilization(mean±standard deviation:76.1%±15.1%vs 65.5%±18.8%),blastocyst formation(55.0%±20.3%vs 30.8%±23.8%),and good morphological quality blastocyst(28.9%±22.9%vs 13.5%±17.9%)and also improvements on pregnancy(60.9%vs 0%)and healthy birth rates(56.5%vs 0%)than EJACULATE cycles.The results described here suggest that in patients with previous IVF/ICSI failures and aberrant mRNA protamine ratios,the use of testicular spermatozoa may be a good alternative to improve clinical outcomes.

关 键 词：DNA damage male infertility mRNA protamine ratio sperm chromatin testicular spermatozoa 

分 类 号：R697[医药卫生—泌尿科学]