miR-16在冠心病患者血清中表达及功能分析  

Expression and function analysis of miR-16 in the serum of patients with coronary heart disease

作　　者：靳星 李文祎 马蓉 Jin Xing;Li Wenyi;Ma Rong(Department of Clinical Laboratory,Longyao County Hospital,Xingtai 055350,China;Department of Clinical Laboratory,Peking University People's Hospital,Beijing 100044,China)

机构地区：[1]隆尧县医院检验科,河北邢台055350 [2]北京大学人民医院检验科,北京100044

出　　处：《临床荟萃》2020年第12期1093-1096,共4页Clinical Focus

摘　　要：目的探讨血浆miR-16与冠心病的关系,并阐明该miR对血管平滑肌细胞增殖表型的调控作用。方法随机选取冠心病患者43例及健康对照49例,通过实时荧光定量聚合酶链反应(PCR)检测血浆miR-16的表达强度。将miR-16类似物转染人血管平滑肌细胞,通过CCK-8实验检测细胞的增殖活力,通过蛋白印迹检测细胞周期蛋白(cyclin)D1、D2和E1的表达。结果冠心病患者血浆miR-16表达水平显著低于对照组(P<0.05)。过表达miR-16可显著抑制血管平滑肌细胞的增殖,并下调cyclin D1、D2和E1的表达。结论miR-16在冠心病患者血浆中表达降低,该miR可能通过靶向抑制细胞周期蛋白cyclinD1、D2和E1的表达抑制血管平滑肌细胞增殖。Objective To explore the relationship between plasma miR-16 and coronary heart disease(CHD),and to clarify the regulation of miR-16 on the proliferation phenotype of vascular smooth muscle cells.Methods Forty-three patients with coronary heart disease and 49 healthy volunteers were randomly selected.The expression intensity of plasma miR-16 was detected by real-time fluorescent quantitative polymerase chain reaction(PCR).miR-16 analogues were transfected into human vascular smooth muscle cells,and the proliferation activity of the cells was detected by CCK-8 experiment.The expression of cyclin D1,D2 and E1 were detected by western blot.Results The expression of plasma miR-16 in CHD patients was significantly lower than that in control group(P<0.05).Overexpression of miR-16 significantly inhibited the proliferation of vascular smooth muscle cells and down-regulated the expression of cyclin D1,D2 and E1.Conclusion miR-16 expression was decreased in plasma of patients with CHD.This miR may inhibit the proliferation of vascular smooth muscle cells through targeting the expression of cyclin D1,D2 and E1.

关 键 词：microRNA-16 冠状动脉疾病 增殖 细胞周期蛋白类 

分 类 号：R541.4[医药卫生—心血管疾病]