槐耳颗粒逆转肝癌侧群细胞耐阿霉素的实验研究  

Reversion of Doxorubicin Resistance of Hepatoma Side Population Cells by Huaier Granule

作　　者：谢正元[1] 赖月亮 刘思甫 汤胜兰 XIE Zheng-yuan;LAI Yue-liang;LIU Si-fu;TANG Sheng-lan(Department of Gastroenterology,the Second Affiliated Hospital,Nanchang University,Nanchang 330006,China;2018 Grade of Department Medicine of Graduate School,Nanchang University,Nanchang 330006,China)

机构地区：[1]南昌大学第二附属医院消化科 [2]南昌大学研究生院医学部,南昌330006

出　　处：《南昌大学学报（医学版）》2020年第5期13-16,21,共5页Journal of Nanchang University：Medical Sciences

基　　金：江西省卫计委中医药科研项目(2017A263)。

摘　　要：目的观察槐耳颗粒对肝癌侧群(side population,SP)细胞阿霉素耐药性的影响。方法采用流式细胞术分离肝癌细胞株MHCC-97L的SP细胞和非SP细胞,分选后的细胞分别经阿霉素(500 ng·mL-1)处理。采用不同质量浓度非细胞毒性剂量(0、2、4、8 mg·mL-1)的槐耳来进行分组实验。MTT法测定细胞增殖活性,并计算细胞抑制率,Western blotting检测耐药转运蛋白P-gp、ABCG2表达水平。结果SP细胞集落形成能力明显强于非SP细胞,形成集落数分别为5.3±1.9、2.2±1.5(P<0.01);槐耳能抑制SP细胞增殖,在质量浓度为10和12 mg·mL-1时更为明显(P<0.05),且在质量浓度为4和8 mg·mL-1时即可增强阿霉素对SP的增殖抑制作用(P<0.05);槐耳能减少耐药转运蛋白P-gp(P<0.05)和ABCG2(P<0.01)的表达。结论槐耳能减少并逆转肝癌SP细胞对阿霉素的耐药性。Objective To observe the effect of Huaier granule on doxorubicin resistance of hepatoma side population(SP)cells.Methods The SP and non-SP cells were isolated from MHCC-97L cell line by flow cytometry,and were treated with doxorubicin(500 ng·mL-1).Furthermore,cells were supplied with non-cytotoxic doses of Huaier(0,2,4,8 mg·mL-1).Cell proliferation was measured by MTT assay to calculate the cell inhibition rate.Western blotting was used to detect the expression of drug resistance transport protein P-gp and ABCG2.Results The number of colonies formed in SP cells(5.3±1.9)was higher than that in non-SP cells(2.2±1.5)(P<0.01).Supplement with Huaier inhibited the proliferation of SP cells at concentrations of 10 and 12 mg·mL-1(P<0.05),and enhanced the inhibition of doxorubicin on SP cells at 4 and 8 mg·mL-1(P<0.05).In addition,Huaier treatment reduced the expression of P-gp(P<0.05)and ABCG2(P<0.01).Conclusion Huaier can reduce and reverse the resistance of hepatoma SP cells to doxorubicin.

关 键 词：槐耳颗粒 侧群细胞 逆转耐药 肝癌 MHCC-97L细胞 阿霉素 

分 类 号：R965[医药卫生—药理学]