滇黄芩醇提物及其不同溶剂萃取部位对CCl4致小鼠肝损伤的保护作用研究  

Study on Protective Effects of Scutellaria amoena Ethanol Extract and Its Different Solvent Parts on CCl4-induced Liver Injury of Mice

作　　者：付胜男 李欣坪 虎春艳 薛咏梅 林玉萍 FU Shengnan;LI Xinping;HU Chunyan;XUE Yongmei;LIN Yuping(School of Chinese Materia Medica,Yunnan University of TCM,Kunming 650500,China)

机构地区：[1]云南中医药大学中药学院,昆明650500

出　　处：《中国药房》2020年第22期2731-2735,共5页China Pharmacy

基　　金：云南省科技计划项目中医联合面上项目[No.2017-FF117(-022)]。

摘　　要：目的:研究滇黄芩醇提物及不同溶剂萃取部位对四氯化碳(CCl4)致小鼠肝损伤的保护作用。方法:用95%乙醇提取滇黄芩得到醇提物浸膏;再经乙酸乙酯、正丁醇依次萃取,得不同溶剂萃取部位。从48只小鼠中随机选取8只为正常组,其余40只为造模组。正常组小鼠腹腔注射给予等体积橄榄油,每3日1次,连续6周;造模组小鼠腹腔注射给予30%CCl4橄榄油溶液,首次剂量为5 mL/kg,以后每次3 m L/kg,每3日1次,连续6周,造成肝损伤模型。将造模成功后的小鼠随机分为模型组(生理盐水)、水飞蓟宾组(阳性对照,20 mg/kg)、滇黄芩醇提物组(100 mg/kg)、滇黄芩乙酸乙酯组(100 mg/kg)、滇黄芩正丁醇组(100 mg/kg),每组8只。每日灌胃1次,连续给药6周。观察各组小鼠实验期间的一般状态;末次给药1 h后,采用酶标仪检测其血清中总胆固醇(TC)、三酰甘油(TG)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)的含量;经苏木精-伊红染色后,观察其肝组织病理形态学变化并进行Ishak评分。结果:正常组小鼠活动正常,毛发浓密有光泽,体质量正常增长,肝组织未见明显病理形态学改变;模型组小鼠精神萎糜,毛发蓬乱无光泽,体质量(给药前及给药第1、2周)均较正常组显著减轻(P<0.05或P<0.01);与正常组比较,模型组小鼠血清中TC、TG、ALT和AST含量均显著升高(P<0.01或P<0.05);肝小叶结构受到破坏严重,炎症细胞浸润严重,肝索组织排列紊乱,Ishak评分显著升高(P<0.01)。与模型组比较,各给药组小鼠上述症状及肝组织病理损伤均有不同程度的改善;水飞蓟宾组、滇黄芩乙酸乙酯组小鼠血清中TC、ALT、AST含量,各给药组小鼠血清中TG含量以及水飞蓟宾组、滇黄芩醇提物组、滇黄芩乙酸乙酯组小鼠肝组织Ishak评分均显著降低(P<0.05或P<0.01)。结论:滇黄芩醇提物及其不同溶剂萃取部位对CCl4致肝损伤模型小鼠均有不同程度的肝保护作用,乙酸乙酯�OBJECTIVE:To study the protective effects of Scutellaria amoena enthanol extract and its different solvent parts on liver injury induced by CCl4.METHODS:S.amoena was extracted with 95%ethanol to obtain ethanol extract,and then was respectively extracted with ethyl acetate and n-butanol to obtain corresponding polar parts.Totally 48 mice were randomly divided into normal group(8 mice)and modeling group(40 mice).Normal group was given constant volume of olive oil intraperitoneally,3 times a day,for consecutive 6 weeks.Model group was given 30%CCl4-olive oil solution intraperitoneally to induce liver injury model,with initial dose of 5 mL/kg after each 3 mL/kg,3 times a days,for 6 consecutive weeks.After modeling,the mice were randomly divided into model group(normal saline),sylibin group(positive control,20 mg/kg),S.amoena ethanol extract group(100 mg/kg),S.amoena ethyl acetate group(100 mg/kg),and S.amoena n-butanol group(100 mg/kg),with 8 mice in each group.After they were given relevant medicine intragastrically once a day,for consecutive 6 weeks.The general information during experiment of mice was observed.1 h after last medication,the serum contents of TC,TG,ALT and AST were determined by Enzyme-labelled meter.After HE staining,the pathological changes of liver tissue were observed and Ishak score was performed.RESULTS:In normal group,mice had normal activity,thick and glossy hair,and the body weight was increased.The liver tissue had no obvious pathological changes.The model group had sparse hair,and they were emaciated and listlessness;and body weight(before medication,1,2 week after medication)was significantly lower than normal group(P<0.05 or P<0.01).Compared with normal group,the contents of TC,TG,ALT and AST in serum were increased significantly(P<0.01 or P<0.05).The structure of hepatic lobule was severely damaged and had more inflammatory cell infiltration;the arrangement of hepatic cord was disordered and the Ishak score was significantly increased(P<0.001).Compared with model group,above symptom an

关 键 词：滇黄芩 醇提物 萃取部位 四氯化碳 肝损伤 小鼠 

分 类 号：R285.5[医药卫生—中药学]