下丘脑外侧区-伏隔核orexin通路对大鼠胃动力及奖赏性摄食的作用  

Effects of LHA-NAc orexinergic pathway on gastric motility and reward feeding in rats

作　　者：刘潇柠 高胜利[1] 张娜娜[2] 金婷婷 孙向荣[1] 郭菲菲[1] Liu Xiaoning;Gao Shengli;Zhang Nana;Jin Tingting;Sim Xiangrong;Guo Feifei(Pathophysiology Department,School of Basic Medicine,Qingdao University f Qingdao 266000,China;Department of Laboratory Medicine,Affiliated Hospital of Qingdao University,Qingdao 266000,China)

机构地区：[1]青岛大学基础医学院病理生理学教研室,266000 [2]青岛大学附属医院检验科,266000

出　　处：《中华行为医学与脑科学杂志》2020年第10期865-872,共8页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目(81500414,31971060,81470815);中国博士后科研基金项目(2018M632627,2018M630749);山东省杰出中青年科学家科研奖励基金项目(BS2014YY009)。

摘　　要：目的:探讨从下丘脑外侧区(LHA)到伏隔核(NAc)的orexin通路对胃动力及奖赏性摄食的调控作用。方法:(1)随机选取48只大鼠,按照随机数字表法分为6组,每组8只,分别为生理盐水组(NS组)、1μg orexin-A组、5μg orexin-A组、10μg orexin-A组、20μg orexin受体拮抗剂(SB334867)组、20μg SB334867+5μg orexin-A组,采用大鼠NAc核团埋置套管注射orexin-A及SB334867的方法,观察大鼠胃收缩的频率和幅度;(2)随机选取32只大鼠,按照随机数字表法分为4组,每组8只,分别为:生理盐水+假刺激组(NS+SS组)、生理盐水+电刺激组(NS+ES组)、SB334867(20μg)+假刺激组(SB334867+SS组)、SB334867(20μg)+电刺激组(SB334867+ES组),采用电刺激大鼠LHA及大鼠NAc注射SB334867的方法,观察大鼠胃收缩的频率和幅度;(3)随机选取32只大鼠,按照随机数字表法分为4组,每组8只,分别为NS组、orexin-A(5μg)组(orexin-A组)、SB334867(20μg)组(SB334867组)、orexin-A(5μg)+SB334867(20μg)组(orexin-A+SB334867组),采用大鼠NAc核团埋置套管注射orexin-A及SB334867的方法,观察大鼠与摄食行为有关的条件性位置偏爱(conditioned place preference,CPP)或者胃功能(比如胃排空或者胃分泌);(4)随机选取32只大鼠,按照随机数字表法分为4组,每组8只,分为生理盐水+假刺激组(NS+SS组)、生理盐水+电刺激组(NS+ES组)、SB334867(20μg)+假刺激组(SB334867+SS组)、SB334867(20μg)+电刺激组(SB334867+ES组),采用电刺激大鼠LHA及大鼠NAc注射SB334867的方法,观察大鼠与摄食行为有关的CPP或者胃功能(比如胃排空或者胃分泌)。结果:(1)胃收缩活动记录结果显示,NAc微量注射orexin-A和电刺激LHA均显著增加了大鼠胃收缩的幅度和频率,并且这种作用可被NAc预注射SB334867阻断[给予orexin-A后10 min:10μg orexin-A组(60.78±5.67)%,NS组(7.35±1.08)%;t=26.18,P<0.05]。(2)胃排空实验结果显示,NAc微量注射orexin-A以及电刺激LHA均可使胃排空的速度显著升高,SB334867预处理Objective:To investigate the regulation of orexinergic pathway from the lateral hypothalamic area(LHA)to the nucleus accumbens(NAc)on gastric function and reward feeding.Methods:(1)Forty-eight rats were randomly selected and divided into six groups:normal saline(NS)group,1μg orexin-A group,5μg orexin-A group,10μg orexin-A group,20μg orexin receptor antagonist(SB334867)group,20μg SB334867+5μg orexin-A group with eight in each group according to the random number table.The gastric motility of rats was observed by injecting orexin-A and SB334867 into NAc.(2)Thirty-two rats were randomly selected and divided into four groups according to the random number table,with eight in each group.They were divided into NS+sham stimulation(SS)group,NS+electrical stimulation(ES)group,SB334867(20μg)+SS group,and SB334867(20μg)+ES group.The gastric motility of rats were observed by electro-stimulation of rat LHA and rat NAc injection of SB334867.(3)In order to observe the feeding-behavior related conditioned place preference(CPP)and gastric function(such as gastric emptying and gastric secretion),thirty-two rats were randomly divided into four groups with eight in each group by using the method of NAc injection of orexin-A and SB334867 according to the random number table:NS group,orexin-A(5μg),SB334867(20μg),SB334867(20μg)+orexin-A(5μg).(4)Thirty-two rats were randomly selected in accordance with the random number table and divided into four groups with eight in each group:NS+SS group,NS+ES group,SB334867(20μg)+SS group,SB334867(20μg)+ES group,using electro-stimulation of rat LHA and rat NAc injection of SB334867,observing the feeding-behavior related CPP and gastric function(such as gastric emptying and gastric secretion).Results:(1)In the gastric motility experiment,both the NAc microinjection of orexin-A and electrical stimulation of the LHA significantly increased the amplitudes and frequencies of gastric contraction in rats,and these effects could be blocked by the pre-administration of SB334867(10 min after adm

关 键 词：OREXIN-A 下丘脑外侧区 伏隔核 胃功能 条件性位置偏爱 摄食 大鼠 

分 类 号：R589.2[医药卫生—内分泌]