刺猬皮提取工艺优化及其提取物TLC鉴别研究  

Study on Extraction Process Optimization and TLC Identification of Corium erinacei

作　　者：周邦华 李本鹏 邓前燕 陈贵霞 王小羽 谭荣 王翔 无 ZHOU Banghua;LI Benpeng;DENG Qianyan;CHEN Guixia;WANG Xiaoyu;TAN Rong;WANG Xiang;无(School of Life and Health Science,Kaili University,Kaili 556011,Guizhou,China;Qiandongnan Engineering and Technology Research Center for Comprehensive Utilization of Ethnic Medicine,Kaili University,Kaili 556011,Guizhou,China;Shenyang Pharmaceutical University,Shenyang 117000,Liaoning,China)

机构地区：[1]凯里学院大健康学院,贵州凯里556011 [2]凯里学院黔东南民族药综合利用工程技术研究中心,贵州凯里556011 [3]沈阳药科大学,辽宁沈阳117000

出　　处：《辽宁中医杂志》2020年第9期119-124,共6页Liaoning Journal of Traditional Chinese Medicine

基　　金：贵州省教育厅青年科技人才成长项目(黔教合KY字[2019]193号);贵州省基础研究计划(科学技术基金)项目(黔科合基础[2020]1Y387);大学生创新创业训练计划项目(LX201943);贵州省全国第四次中药资源丹寨县普查专项(HX201805);黔东南州国家火炬苗侗医药特色产业基地项目(黔东南科合基地J字[2018]007号)。

摘　　要：目的优选出刺猬皮的最佳工艺参数并建立其提取物的TLC法。方法以总氨基酸含量和干膏得率为指标,通过单因素试验,结合正交试验-综合评分法考察乙醇浓度,料液比,提取时间及提取次数对提取工艺影响;同时以谷氨酸,甘氨酸,天门冬氨酸为鉴别指标,对刺猬皮提取物进行TLC鉴别。结果最佳提取工艺为:取刺猬皮炭品粉末,料液比为1∶14,70%乙醇提取120 min,提取1次;该提取物的TLC鉴别斑点清晰,分离度较好。结论该实验优选的提取工艺参数稳定、可靠,可为刺猬皮制剂制备提供实验参考。Objective To optimize the technological parameters of Corium erinacei and establish TLC for its extract. Methods By using total amino acid content and dry paste yield as indexes,the effects of ethanol concentration,solid-liquid ratio,extraction time and extraction times on extraction process were investigated by single factor test and orthogonal test-comprehensive scoring method. Meanwhile,glutamic acid,glycine and aspartic acid were used as identification indexes to identify the extracts from Corium erinacei by TLC. Results The best extraction process was as follows: the charcoal powder of Corium erinacei was extracted by70% ethanol for 120 min with the ratio of material to liquid being 1: 14. The TLC of the extract showed clear spots and good separation. Conclusion The optimized extraction parameters are stable and reliable. This method can provide experimental reference for preparation of Corium erinacei.

关 键 词：刺猬皮 UV TLC 正交试验 工艺参数 

分 类 号：R284[医药卫生—中药学]