传统抗疟中药青蒿-常山抗过早搏动的网络药理学研究  

Mechanism of Traditional Anti-Malarial Chinese Medicine Qinghao(Artemisia annua)-Changshan(Dichroae febrifuga) for Treating Premature Beat Based on Network Pharmacology

作　　者：张永健 陈纪烨 王永成 周国锋 林芮 马跃[1] 张星[1] 李晓[2] ZHANG Yongjian;CHEN Jiye;WANG Yongcheng;ZHOU Guofeng;LIN Rui;MA Yue;ZHNAG Xing;LI Xiao(Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong,China;Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250011,Shandong,China)

机构地区：[1]山东中医药大学,山东济南250014 [2]山东中医药大学附属医院,山东济南250011

出　　处：《辽宁中医杂志》2020年第9期128-132,I0002,I0003,共7页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81673970);全国名老中医药专家丁书文传承工作室建设项目(国中医药人教发[2010]59号)。

摘　　要：目的基于网络药理学的研究方法探讨传统抗疟中药青蒿-常山抗过早搏动(premature beat)的作用机制。方法运用中药系统药理数据库和分析平台(TCMSP数据库)和相关文献检索并筛选青蒿-常山活性化学成分及相关靶点蛋白,通过Uniport数据库将靶点蛋白名转化为靶点基因名。构建青蒿和常山活性化学成分-靶点网络。在Gene Cards和OMIM数据库检索过早搏动疾病的相关靶点基因,并构建疾病-靶点网络。筛选出青蒿-常山活性化学成分直接作用于过早搏动疾病的靶点。将直接作用靶点输入STRING数据库进行蛋白相互作用,获得并分析蛋白互作网络;将直接作用靶点输入DAVID数据库进行GO功能和KEGG通路富集分析。结果筛选出青蒿22个活性化学成分,常山18个活性化学成分。获得青蒿靶点蛋白510个,常山靶点蛋白202个,通过Uniport数据库转化为基因名并去除重复获得青蒿-常山靶点基因110个。在OMIM,Gene Cards数据库中筛选出过早搏动疾病靶点基因971个。进一步筛选出青蒿-常山直接作用靶点43个。通过STRING数据库的蛋白互作网络,筛选出关键靶点。运用DAVID数据库的GO功能分析获得244个富集结果,涉及生物过程、细胞组分、分子功能;KEGG分析获得50个相关通路。结论通过网络药理学对传统抗疟中药青蒿-常山的研究表明,青蒿-常山可通过多成分-多靶点-多通路对过早搏动具有治疗作用,为进一步解释和阐述青蒿常山抗过早搏动可能的作用机制奠定了基础。Objective To study the mechanism of traditional anti-malarial Chinese medicine Qinghao( Artemisia annua)-Changshan( Dichroa febrifuga) against premature beat based on network pharmacology research. Methods The TCMSP database and related literature were searched and the active chemical compounds of Qinghao( Artemisia annua)-Changshan( Dichroa febrifuga) and related target proteins were screened,and the target protein name was transformed into the target gene name by the Uniport database. We constructed active chemical compounds of Qinghao( Artemisia annua)-Changshan( Dichroa febrifuga)-Target Networks. We searched the relevant target genes for premature beat disease in Gene Cards and OMIM databases and built disease-targets networks. We screened the active chemical compounds of Qinghao( Artemisia annua)-Changshan( Dichroa febrifuga) directly which affect the targets of premature beat disease,input the directly targets into the STRING database for protein interaction,obtained and analyzed the protein interaction network. And then we input the directly targets into the DAVID database for GO function and KEGG pathway analysis. Results A total of 22 active chemical compounds of Qinghao( Artemisia annua) and18 active chemical compounds of Changshan( Dichroae febrifuga) were screened. 510 target proteins of Qinghao( Artemisia annua) and 202 target proteins of Changshan( Dichroa febrifuga) were obtained,which were transformed into gene names by Uniport database and 100 genes of Qinghao( Artemisia annua)-Changshan( Dichroa febrifuga) target genes were obtained by repeated removal. In the OMIM,Gene Cards database,971 target genes for premature beat disease were screened. A total of 43 direct targets of Qinghao( Artemisia annua)-Changshan( Dichroa febrifuga) were screened. Key targets were screened through the protein interaction network of the STRING database. By using the GO function analysis of the DAVID database,244 enrichment results were obtained,involving biological processes,cellular components,and molecular functio

关 键 词：青蒿 常山 过早搏动 网络药理学 通路 作用机制 

分 类 号：R541.7[医药卫生—心血管疾病]