穴位电针刺激对烫伤致大鼠急性肺损伤炎症指标的影响  

Effects of Electro-acupuncture Stimulation at Acupoints on Inflammatory Indexes in Burn-Induced Acute Lung Injury Rats

作　　者：王爱群 殷文慧 曹拓 王鹏珍 WANG Ai-Qun;YIN Wen-Hui;CAO Tuo;WANG Peng-Zhen(Dept.of Anesthesiology,Guangzhou Red Cross Hospital Affiliated to Jinan University,Guangzhou 510220 Guangdong,China;Dept.of Cardiology,Guangzhou Red Cross Hospital Affiliated to Jinan University,Guangzhou 510220 Guangdong,China;Dept.of Laboratory,Guangzhou Red Cross Hospital Affiliated to Jinan University,Guangzhou 510220 Guangdong,China;Guangzhou Institute of Traumatic Surgery,Guangzhou 510220 Guangdong,China)

机构地区：[1]暨南大学附属广州红十字会医院麻醉科,广东广州510220 [2]暨南大学附属广州红十字会医院心血管科,广东广州510220 [3]暨南大学附属广州红十字会医院检验科,广东广州510220 [4]广州市创伤外科研究所,广东广州510220

出　　处：《广州中医药大学学报》2020年第11期2134-2139,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药局科研项目(编号:20201076);广州市中医药和中西医结合科技资助项目(编号:20192A010009);广州市卫生健康科技一般引导项目(编号:20191A011018)。

摘　　要：【目的】探讨穴位电针刺激对烫伤致急性肺损伤大鼠炎症指标的影响。【方法】将24只健康雄性SD大鼠随机分为4组,即正常组、模型组、穴位电针组和非穴位电针组,每组各6只,除正常组外,其余各组均制备大鼠烫伤模型。造模后,穴位电针组选取大鼠足三里和肺俞穴,给予电针刺激,非穴位电针组选取足三里和肺俞穴周围固定部位进行电针刺激,均连续进行1周。分别测定各组大鼠动脉血氧分压(PaO2)和二氧化碳分压(PaCO2),采用ELISA法测定血清超氧化物歧化酶(SOD)、肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)含量,并检测大鼠肺组织裂解液中一氧化氮合酶(iNOS)和环氧化酶(COX2)的含量。【结果】穴位电针组大鼠动脉血中Pa O2显著升高,PaCO2显著降低,与模型组比较,差异均有统计学意义(P<0.05);而非穴位电针组未能显著改变大鼠PaO2和PaCO2,与模型组比较,差异无统计学意义(P>0.05)。穴位电针组大鼠血清SOD水平显著升高,血清TNF-α和IL-6水平均显著降低,与模型组比较,差异有统计学意义(P<0.05)。而非穴位电针组未能显著改变大鼠血清SOD、TNF-α和IL-6水平,与模型组比较,差异均无统计学意义(P>0.05)。穴位电针组大鼠肺组织iNOS和COX2水平显著降低,与模型组比较,差异均有统计学意义(P<0.05);而非穴位电针组未能显著改变大鼠肺组织iNOS和COX2水平,与模型组比较,差异无统计学意义(P>0.05)。【结论】穴位电针刺激可降低烫伤急性肺损伤大鼠炎症指标水平,具有抗肺损伤作用。Objective To explore the effects of electro-acupuncture stimulation at acupoints on inflammatory factors in burn-induced acute lung injury rats.Methods Twenty-four healthy male SD rats were randomly divided into normal group,model group,acupoint electro-acupuncture group and non-acupoint electro-acupuncture group,6 rats in each group.Apart from the normal group,the rats in the other groups were established into burn model.After modeling,the acupoint electro-acupuncture group received electro-acupuncture at Zusanli(ST36)and Feishu(BL13)points,non-acupoint electro-acupuncture group received electro-acupuncture at fixed sites around Zusanli(ST36)and Feishu(BL13)points,the treatment lasting one week.The PaO2 and PaCO2 were measured in various groups,and the contents of superoxide dismutase(SOD),tumor necrosis factorα(TNF-α)and interleukin 6(IL-6)in serum and contents of inducible nitric oxide synthase(iNOS)and cyclooxygenase 2(COX2)in lung tissues were determined by enzyme-linked immunosorbent assay(ELISA).Results Compared with the model group,PaO2 was increased and PaCO2 was decreased in the acupoint electro-acupuncture group,the differences being significant(P<0.05),PaO2 and PaCO2 were not obviously changed in the non-acupoint electroacupuncture group,the differences being insignificant(P>0.05).Compared with the model group,the serum SOD level was increased,and serum TNF-αand IL-6 levels were decreased in the acupoint electro-acupuncture group,the differences being significant(P<0.05),serum SOD,TNF-αand IL-6 levels were not obviously changed in the non-acupoint electro-acupuncture group,the differences being insignificant(P>0.05).Compared with the model group,the levels of iNOS and COX2 in lung tissues were decreased in the acupoint electroacupuncture group,the differences being significant(P<0.05),and the levels of iNOS and COX2 in lung tissues were not obviously changed in the non-acupoint electro-acupuncture group,the differences being insignificant(P>0.05).Conclusion Electro-acupuncture stimulation at acupo

关 键 词：电针 穴位刺激 烫伤 急性肺损伤 炎症因子 大鼠 

分 类 号：R246.9[医药卫生—针灸推拿学]