微水固相法对皂荚多糖的阳离子化改性工艺研究  

Cationic Modification of Gleditsia Polysaccharide by Microaqueous Solid Phase Method

作　　者：朱昌玲 雷鹏 张锋伦 ZHU Changling;LEI Peng;ZHANG Fenglun(Nanjing Institute for Comprehensive Utilization of Wild Plant China CO-OP,Nanjing 211111,China)

机构地区：[1]中华全国供销合作总社南京野生植物综合利用研究所,江苏南京211111

出　　处：《生物质化学工程》2020年第6期53-58,共6页Biomass Chemical Engineering

基　　金：国家重点研发计划资助项目(2016YFD0600803)。

摘　　要：以皂荚种子胚乳片为原料,以3-氯-2-羟丙基三甲基氯化铵为醚化剂,采用微水固相工艺制备了阳离子皂荚多糖,通过单因素试验考察了氢氧化钠质量分数、醚化剂质量分数、反应温度及反应时间对产物黏度和取代度的影响,确定了最优反应条件为皂荚种子胚乳片500 g、250 mL 40%NaOH溶液、500 mL 60%的3-氯-2-羟丙基三甲基氯化铵溶液、反应温度60℃和反应时间4 h。该条件下制备的阳离子皂荚多糖含水不溶物为2.81%、黏度为1196.2 mPa·s(1%的水溶液,25℃)、阳离子取代度为0.151。红外光谱和核磁共振氢谱的表征结果均证明皂荚多糖实现了季铵型阳离子化反应,阳离子皂荚多糖的表观黏度测试结果显示其符合皂荚多糖的假塑性流体特征。The endosperm slices of Gleditsia sinensis seed were used as raw materials to prepare cationic Gleditsia polysaccharide by microaqueous solid phase method with 3-chloro-2-hydroxypropyl trimethyl ammonium chloride as the etherifying agent.And the effects of preparation conditions,including the sodium hydroxide dosage,etherifying agent dosage,reaction temperature and reaction time on the viscosity and substitution degree of the product were studied by single factor experiment.The optimal reaction conditions were determined as endosperm slices of Gleditsia sinensis seed 500 g,250 mL 40%NaOH solution,500 mL 60%3-chloro-2-hydroxypropyl trimethyl ammonium chloride solution,reaction temperature 60℃and the reaction time of 4 h.The content of water insoluble substance of cationic Gleditsia polysaccharide was 2.81%,the viscosity was 1196.2 mPa·s(1%water solution at 25℃),and the degree of cation substitution was 0.151.Infrared spectrum and nuclear magnetic resonance results showed that the quaternized reaction of Gleditsia sinensis polysaccharide was realized,the viscosity test of cationic Gleditsia polysaccharide also accorded with the pseudoplastic fluid characteristics.

关 键 词：微水固相法 阳离子 皂荚多糖 工艺 

分 类 号：TQ35[化学工程] TQ311