桂枝茯苓丸对大鼠肝脏纤维化的防治作用及其对肝组织病理学形态的影响  

Study on Prevention and Treatment by using Guizhi Fuling Pills in Liver Fibrosis of Rats and Its Influence on Histopathological Morphology

作　　者：李小红[1] 叶军[2] 王佩 LI Xiao-hong;YE Jun;WANG Pei(The Infectious Diseases Hospital Affiliated to Soochow University(The No.5 People's Hospital of Suzhou),Suzhou Jiangsu 215000,China;Putuo Hospital Affiliated to Shanghai University of TCM,Shanghai 200062,China)

机构地区：[1]苏州大学附属传染病医院(苏州市第五人民医院),江苏苏州215000 [2]上海中医药大学附属普陀医院,上海200062

出　　处：《抗感染药学》2020年第10期1411-1416,共6页Anti-infection Pharmacy

摘　　要：目的:探究桂枝茯苓丸对大鼠肝脏纤维化的保护作用及组织病理学形态的影响以及可能的作用机制。方法:将88只大鼠随机分为正常组(8只)、模型组(20只)、桂预组(20只)、桂治组(20只)和秋治组(20只);正常组大鼠给予生理盐水灌胃,桂预组在给予桂枝茯苓丸汤剂同时给予造模剂,其余各组大鼠造模6周后,分别给予秋水仙碱(秋治组)、生理盐水(模型组)、桂枝茯苓丸汤剂(桂治组)治疗4周、10周后于大鼠肝左叶的相同部位用刀片切取肝组织,将其用于HE、Masson染色法观察其肝脏组织病理学形态的变化;以及采用免疫组化法检测MMP-9、TIMP-1及TGF-β1的表达水平。结果:正常组大鼠肝组织细胞形态呈正常、排列整齐,未见肝纤维化改变;而模型组肝组织细胞变性、排列紊乱,可见不同程度肝纤维化改变,模型组最明显,桂治组、秋治组大鼠肝脏细胞变性较模型组轻;桂预组与秋治组比较,肝细胞变性不明显、排列规则,肝纤维化轻度增生;采用免疫组化研究结果显示,模型组TIMP-1、TGF-β1阳性表达高于正常组(P<0.01);桂治组大鼠TIMP-1、TGF-β1阳性表达低于模型组(P<0.05);桂预组大鼠TIMP-1、TGF-β1阳性表达低于桂治组、秋治组(P<0.01);桂治组与秋治组比较其差别无统计学意义(P>0.05);模型组大鼠MMP-9蛋白表达高于正常组(P<0.001);桂治组与秋治组大鼠MMP-9蛋白表达低于模型组(P<0.05);桂预组大鼠MMP-9蛋白表达低于桂治组(P<0.05);而桂治组与秋治组之间的差异无统计学意义(P>0.05)。结论:桂枝茯苓丸可降低肝纤维化程度,预防使用效果更为明显,其抑制肝纤维化的作用可能与下调TIMP-1、TGF-β1阳性的表达有关。Objective:To explore the prevention and treatment by using Guizhi Fuling pills on liver fibrosis of rats,and to study its influence on histopathological morphology and the possible mechanism of action.Methods:88 rats were randomLy divided into normal group(8 rats),model group(20 rats),GUI pregroup(20 rats),GUI treatgroup(20 rats)and Qiu treatgroup(20 rats).Normal group rats were given normal saline to filling the stomach,groups building 6 weeks later,the rats were given colchicine(Qiu treatgroup),normal saline group(model group),GUI pregroup by Guizhi Fuling pills(GUI treatgroup)in rats after treatment of 4 weeks,10 weeks left hepatic lobe in the same place with the blade cut in liver tissue,used it for HE and Masson staining to observe the changes in the form of liver tissue pathology.The expression levels of MMP-9,TIMP-1 and TGF-β1 were detected by immunohistochemistry.Results:In the normal group,the morphology of liver cells was normal,and the liver fibrosis was not observed.In the model group,the liver cells were denaturated and arranged in disorder,showing different degrees of changes in liver fibrosis;the rats of model group are the most obvious.The liver cell degeneration of rats was treated with Guizhi Fuling pills and colchicine was lighter than that of the model group.Compared with the treatment groups,the cell of preventive treatment group of Guizhi Fuling pills degeneration were not obvious,liver cell arrangement was regular,and liver fibrosis was mild hyperplasia.Immunohistochemical results showed that the positive expression of TIMP-1 and TGF-β1 in the model group were more than that in the normal group,with a significant difference(P<0.01).Compared with the model group,the positive expressions of TIMP-1 and TGF-β1 in the treatment group were less(P<0.05 or P<0.01).Compared with Guizhi Fuling pills treatment group and colchicine treatment group,the positive expression of timp-1 TGF-β1 in Guizhi Fuling pills preventive treatment group was significantly reduced(P<0.01).There was no significant dif

关 键 词：桂枝茯苓丸 防治 肝纤维化 作用机制 

分 类 号：R285[医药卫生—中药学]