叶用芥菜抽薹速率与现蕾期生理生化特征的关系  

Relationship Between Bolting Speed and Physiological and Biochemical Characteristics at Bolting Stage in Leaf Mustard

作　　者：严奉君 刘独臣[1,2] 代云璐 巩雪峰 常伟[1,2] 李跃建 YAN Fengjun;LIU Duchen;DAI Yunlu;GONG Xuefeng;CHANG Wei;LI Yuejian(Institute of Horticulture,Sichuan Academy of Agricultural Sciences/Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province,Chengdu,Sichuan 610066;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Southwest Region)Ministry of Agriculture,Chengdu,Sichuan 610066;People's Government of Putou Town,Lixian County,Aba Autonomous Prefecture,Lixian,Sichuan 623100)

机构地区：[1]四川省农业科学院园艺研究所,蔬菜种质与品种创新四川省重点实验室,四川成都610066 [2]农业部西南地区园艺作物生物学与种质创制重点实验室,四川成都610066 [3]阿坝州理县朴头镇人民政府,四川理县623100

出　　处：《北方园艺》2020年第21期27-33,共7页Northern Horticulture

基　　金：四川省科技厅应用基础资助项目(2020YJ0468);国家产业技术体系资助项目(CARS-24-G-19);四川省财政专项资金资助项目(2016CYTS-008)。

摘　　要：以3个不同耐抽薹性芥菜材料F7212、F7073与F7170为研究对象,采用调查各材料抽薹特性及分析芥菜现蕾前0~4 d叶片生理生化变化特征进行综合分析评价的方法,研究了芥菜现蕾期叶片生理生化指标的变化特征以及与抽薹速率的相互关系,以期揭示芥菜现蕾期生理生化特征以及为抽薹特性的相互关系研究提供了参考依据。结果表明:各芥菜材料在现蕾前0~4 d叶片可溶性蛋白质含量、过氧化物酶(POD)、超氧化物歧化酶(SOD)与过氧化氢酶(CAT)活性均呈逐渐上升的趋势,而可溶性糖含量则逐渐降低。在各时期叶片可溶性蛋白质含量与CAT活性均随芥菜抽薹速率的降低而增加,以F7212最高,分别较其它材料提高0.12%~0.91%与14.54%~149.86%。SOD活性则随芥菜抽薹耐性的增加而降低,F7212分别较其它材料降低2.68%~116.00%。此外低抽薹速率芥菜材料(F212)在可溶性蛋白质含量与SOD活性方面具有大跨度的上升幅度,而在POD以及CAT活性上则表现为平稳的上升。相关研究表明芥菜抽薹速率与POD活性、CAT活性有显著相关关系,而与可溶性蛋白质、可溶性糖含量及SOD活性无显著相关关系。通径分析表明,可溶性糖与SOD活性对芥菜抽薹速率直接作用最大(-0.80~1.09),但可溶性蛋白质含量、POD活性与CAT活性间接作用于可溶性糖含量及SOD活性,表明芥菜叶片SOD活性升高以及叶片可溶性糖快速向外部转移是加速芥菜抽薹速率的重要因素,而植株体内稳定及活跃POD与CAT能够显著减缓芥菜抽薹速率。Three mustard materials with different bolting tolerance,F7212,F7073 and F7170 were selected in this study,the physiological and biochemical characteristics of leaves in 0-4 days before bolting stage were identified,and the correlation between each index and the bolting speed of mustard were studied,in order to provide a theoretical basis for revealing the physiological and biochemical characteristics of mustard buds and their relationship with bolting tolerance.The results showed that the activities of soluble protein,POD,SOD and CAT in the leaves of various mustard were performed gradually increasing during the 0-4 days before bolting stage,while the soluble sugar decreased.Meanwhile,the soluble protein content and CAT activity in leaves increased with the decline in bolting speed.Compared with other materials,F7212 was the highest on the soluble protein content and CAT activity,which increased by 0.12%-0.91% and 14.54%-149.86% respectively.However,SOD activity decreased with the increase of bolting tolerance,F7212 decreased by 2.68%-116.00% respectively.In addition,the soluble protein content and SOD activity of the low bolting speed mustard material performed sharply increasing.While,the POD and CAT activities showed a steady increasing.Correlation study showed that there was a significant correlation between the bolting speed and POD and CAT activities,but no significant correlation with soluble protein,soluble sugar contents and SOD activity.Path analyses showed that soluble sugar content and SOD activity were the most direct effects on the bolting speed(-0.80-1.09),but soluble protein,POD and CAT indirectly effected on soluble sugar and SOD.It Indicated that with the SOD increasing,the soluble sugar in leaf quickly transfer to outside were main the important factors to accelerate the mustard bolting rate.

关 键 词：芥菜 现蕾期 过氧化物酶 过氧化氢酶 超氧化物歧化酶 抽薹速度 

分 类 号：S637.2[农业科学—蔬菜学]