茶陵野生稻种子休眠性QTL定位  

QTL Mapping for Seed Dormancy in Chaling Common Wild Rice

作　　者：郑传琴 江南 曾苗春 赵新辉 吴钟秀 李艳锋 严天泽 傅军 周延彪 王凯[1,2,3] 杨广 杨远柱[1,2,3,4] ZHENG Chuan-qin;JIANG Nan;ZENG Miao-chun;ZHAO Xin-hui;WU Zhong-xiu;LI Yan-feng;YAN Tian-ze;FU Jun;ZHOU Yan-biao;WANG Kai;YANG Guang;YANG Yuan-zhu(Yahua Seeds Science Academy of Hunan,Changsha 410604;Yuan Longping High-Tech Agriculture Co.,Ltd/Kwy Laboratory of Southern Rice Innovation&Improvement,Ministry of Agriculture and Rural Affairs/Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding,Changsha 410128;College of Life Sciences,Hunan Normal University Changsha 410081;College of Agronomy,Hunan Agricultural University,Changsha 410128)

机构地区：[1]湖南亚华种业科学研究院,长沙410604 [2]袁隆平农业高科技股份有限公司/农业农村部南方水稻品种创制重点实验室/抗病虫水稻育种湖南省工程实验室,长沙410128 [3]湖南师范大学生命科学学院,长沙410081 [4]湖南农业大学农学院,长沙410128

出　　处：《植物遗传资源学报》2020年第6期1521-1526,共6页Journal of Plant Genetic Resources

基　　金：国家重点研发计划(2018YFD0100900);杂交水稻国家重点实验室(湖南杂交水稻研究中心)开放课题基金(2018KF03);国家自主创新示范区专项(2018XK2005);湖南省科技创新计划(2018NK1020)。

摘　　要：我国南方地区水稻(Oryza sativa L.)在成熟收获季节常遇连续高温多雨天气,易出现穗发芽现象,严重影响稻米产量、品质、稻谷贮藏和稻种质量。种子适度的休眠性可避开不良环境对种子萌发的影响,避免或减轻穗发芽危害。因此,开展水稻种子休眠性遗传机制研究,对培育抗穗发芽水稻品种,保障我国水稻生产与种子产业的发展具有重要意义。本研究以籼稻品种93-11为受体和轮回亲本,茶陵野生稻(O.rufipogon Griff.)为供体亲本构建的高代回交渗入系群体为材料,进行种子休眠性鉴定,获得5个表现较强休眠性的单株,发芽率为10.0%~36.5%,而对照及轮回亲本93-11发芽率为89.5%。5个强休眠单株自交后的株系平均发芽率为3.1%~17.4%,进一步利用其中3个株系的强休眠后代单株,分别与93-11杂交构建F2群体,通过BSA(Bulked segregant analysis)方法,结合56 K全基因组SNP芯片数据,共检测到8个QTL,分别位于水稻第3、5、6、10号染色体上。本研究为进一步精细定位和克隆种子休眠性QTL以及培育抗穗发芽水稻新品种奠定了理论和材料基础。In southern China,rice(Oryza sativa L.)grains are prone to field pre-harvest sprouting(PHS)due to high temperature and humid weather conditions,which seriously affects the yield,quality and storage of rice.Moderate seed dormancy prevents the adverse effects of the unfavorable environment and helps reduce or avoid PHS.Therefore,genetic studies of seed dormancy and breeding PHS-resistant varieties,are extremely important for rice production and development of seed industry.In this study,we used the indica cultivar’93-11’as the recipient and recurrent parent,and Chaling common wild rice(CLCW)of O.rufipogon Griff.as the donor parent to develop an advanced backcross introgression population consisting of 812 lines.We assessed the seed dormancy of the population,and found five individual plants displaying strong dormancy with the germination rate ranging from 10.0%to 36.5%,while the control and recurrent parent’93-11’had the germination rate of 89.5%.The germination rate of the selfed lines derived from the five strong-dormancy plants ranged from 3.1%to 17.4%.The plants with extremely strong seed dormancy selected from each of 3 selfed lines were crossed with’93-11’to develop the F2 population.By combining the bulked segregant analysis(BSA)and 56 K SNP chip data,8 QTLs were detected,distributed on chromosomes 3,5,6,and 10.This study will facilitate further mapbased cloning of these QTLs and breeding PHS-resistant rice varieties.

关 键 词：茶陵野生稻 种子休眠性 SNP芯片 QTL 

分 类 号：S511.9[农业科学—作物学]