弯刺蔷薇枝条越冬抗寒生理与蛋白组学分析  

Physiological and Proteomics Analysis on Freezing Tolerance of Rosa beggeriana Branches during Overwintering

作　　者：徐远 杨树华[1] 贾瑞冬[1] 赵鑫[1] 何连球 孟文慧 周苗新 葛红[1] XU Yuan;YANG Shu-hua;JIA Rui-dong;ZHAO Xin;HE Lian-qiu;MENG Wen-hui;ZHOU Miao-xin;GE Hong(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences/National Flowers Improvement Center of China,Beijing 100081;Beijing Xushixinhua Agricultural Technology Limited Company,Beijing 102104;Bureau of Agriculture and Rural Affairs of Xincheng District,Huhhot City,Inner Mongolia Autonomous Region,Huhhot 010010;Garden&Landscaping Center of Xintai City,Shangdong Province,Taian 271200)

机构地区：[1]中国农业科学院蔬菜花卉研究所/国家花卉改良中心,北京100081 [2]北京旭世欣华农业科技有限公司,北京102104 [3]内蒙古自治区呼和浩特市新城区农牧水利局,呼和浩特010010 [4]山东省新泰市园林绿化中心,泰安271200

出　　处：《植物遗传资源学报》2020年第6期1568-1576,共9页Journal of Plant Genetic Resources

基　　金：国家重点研发计划课题(2019YFD1001001);北京市科委科技计划课题(Z191100008519003);中央级公益性科研院所基本科研业务费专项(Y2017PT52,Y2018YJ19,Y2019YJ11);农业农村部园艺作物生物学与种质创制重点实验室。

摘　　要：以弯刺蔷薇(Rosa beggeriana Schrenk ex Fisch.&C.A.Mey.)为试验材料,测定其当年生枝条在初冬阶段至严冬阶段低温半致死温度(LT50),并在测定类黄酮、非结构性碳水化合物以及植物激素脱落酸(ABA)、水杨酸(SA)含量变化的基础上,对2个阶段的枝条开展蛋白质组学分析。结果发现:随着气温的降低,弯刺蔷薇枝条抗寒性逐渐增强,其LT50值在严冬阶段达到-54.7℃。枝条中淀粉和ABA含量无显著变化,类黄酮含量、总可溶性糖及其主要组分蔗糖、葡萄糖、果糖、棉籽糖含量显著升高,SA含量则呈现出先下降再上升的变化。相关性分析表明,LT50值与类黄酮、总可溶性糖及其主要组分葡萄糖、果糖含量存在显著负相关。进一步的蛋白质组学分析发现,枝条在越冬过程中发现了大量差异表达蛋白,在初冬阶段和严冬阶段分别为92个和91个,其中2个阶段均存在表达差异蛋白18个。越冬期间,淀粉和蔗糖代谢、类黄酮生物合成、抗氧化系统、细胞壁松弛与延伸、水分运输通道、细胞脱水耐受等相关蛋白显著上调,表明弯刺蔷薇枝条启动了一系列的生理生化变化以响应长期冷冻低温胁迫。特别是进入严冬阶段,脱水素、抗冻蛋白和水通道蛋白可能为维持细胞水分状况发挥了重要作用。本研究为深入揭示弯刺蔷薇耐寒分子机制打下了坚实基础。This study investigated the changes in half lethal low temperature(LT50),the contents of flavonoid,non-structural carbohydrates and plant hormone,as well as the proteomics on the current-year branches of Rosa beggeriana Schrenk ex Fisch.&C.A.Mey.during overwintering.The results showed that the freezing tolerance of the branches gradually increased and its LT50 value reached-54.7℃in the coldest phase of the winter.No significance on the contents of starch and ABA were observed,whereas the contents of flavonoids,total soluble sugars including sucrose,glucose,fructose and raffinose were found to be significantly increased in the branches.However,SA contents in the branches decreased at the beginning phase of the winter,followed by an increase significantly in the coldest phase of the winter.The correlative analysis showed that the LT50 values were negatively correlated with the contents of flavonoids,total soluble sugars,glucose and fructose.The proteomic analysis showed that there were total 153 differentially expressed proteins(DEPs)accumulated in branches during overwintering.Of them,there were 92 DEPs at the beginning phase of the winter and 91 DEPs in the coldest phase of the winter.The DEPs,which are annotated to involve in starch and sucrose metabolism,flavonoids biosynthesis,antioxidant defenses,cell wall relaxation and extension,water transport channels as well as cell dehydration responses were significantly up-regulated,suggesting that the branches activated a series of physiological and biochemical responses to improve the long-term freezing tolerance.Moreover,the DEPs such as dehydrin,anti-freezing protein and aquaporin may play the important roles in the cell water balances of the branches in the coldest phase of the winter.

关 键 词：弯刺蔷薇 枝条 抗寒性 可溶性糖 蛋白质组学 脱水素 

分 类 号：S685.12[农业科学—观赏园艺]