獭兔源大肠杆菌和巴氏杆菌的混合感染及耐药性鉴定  

Mixed Infection and Drug Resistance of E.coli and Pasteurella from Rax Rabbits

作　　者：乔国峰[1] 孙乐天[2] 张国权[1] 王志宇[1] 任克良[1] 薛俊龙[1] 王国艳[1] Qiao Guo-feng;Sun Le-tian;zhang Guo-quan;Wang Zhi-yu;Ren Ke-liang;Xue Jun-long;Wang Guo-yan(Sciences of ShanXi Agricultural Academy Institute of Animal Husbandry and Veterinary,TaiYuan 030032;Tai Yuan Zoo,TaiYuan 030009)

机构地区：[1]山西省农业科学院畜牧兽医研究所,太原030032 [2]山西省太原动物园,太原030009

出　　处：《中国养兔》2020年第5期12-14,24,共4页Chinese Journal of Rabbit Farming

基　　金：山西省农业科学院特色农业技术攻关项目(YGG17092);国家兔产业技术体系专项资金资助(CARS-43-B-3)。

摘　　要：2018年11月末,山西南部某獭兔养殖场突发以腹泻和呼吸道症状为主的高致死疾病。通过对无菌采集的病死兔肺脏、胃肠道等29份病料进行实验室常规细菌培养;再通过16s rDNA诊断方法确定分离到29株大肠杆菌和20株巴氏杆菌;选取12种常用抗生素,采用K-B纸片法进行药敏试验。结果显示,兔源大肠杆菌和巴氏杆菌对磺胺甲氧嘧啶、庆大霉素的耐药率较高,对氟苯尼考、氧氟沙星较为敏感,对其他的抗生素均表现出现不同程度的耐药性。At the end of November 2018,a rex rabbit farm in the south of Shanxi Province was suddenly suffered from the high death rate diseases mainly caused by diarrhea and respiratory symptoms.The 29 samples of disease materials,including the lung and gastrointestinal tract,collected aseptically,were routinely cultured in the laboratory.Then 29 strains of escherichia coli and 20 strains of pasteurella were isolated by 16 S rDNA diagnosis.Seven commonly used antibiotics were selected and tested by k-B paper method.The results showed that rabbit-derived escherichia coli and pasteurella had higher drug resistance rates to gentamicin sulfamethoxine,and were more sensitive to flumphenicol,ofloxacin,norfloxacin and showed the varying degrees of resistance to other antibiotics.

关 键 词：细菌耐药 大肠杆菌 巴氏杆菌 16s rDNA鉴定 

分 类 号：S829.1[农业科学—畜牧学]