Delayed atomoxetine or fluoxetine treatment coupled with limited voluntary running promotes motor recovery in mice after ischemic stroke  

作　　者：Faisal F.Alamri Abdullah Al Shoyaib Nausheen Syeara Anisha Paul Srinidhi Jayaraman Serob T.Karamyan Thiruma V.Arumugam Vardan T.Karamyan 

机构地区：[1]Department of Pharmaceutical Sciences,School of Pharmacy,Texas Tech University Health Sciences Center,Amarillo,TX,USA [2]Department of Pharmacology,Faculty of Pharmacy,Yerevan State Medical University,Yerevan,Armenia [3]Department of Physiology,Anatomy and Microbiology,School of Life Sciences,La Trobe University,Melbourne,Australia [4]Center for Blood Brain Barrier Research,School of Pharmacy,Texas Tech University Health Sciences Center,Amarillo,TX,USA [5]College of Sciences and Health Profession,King Saud bin Abdulaziz University for Health Sciences and King Abdullah International Medical Research Center,Jeddah,Saudi Arabia

出　　处：《Neural Regeneration Research》2021年第7期1244-1251,共8页中国神经再生研究（英文版）

基　　金：partly supported by a National Institutes of Health Research Grant to VTK (1R01NS106879)。

摘　　要：Currently, there is an unmet need for treatments promoting post-stroke functional recovery.The aim of this study was to evaluate and compare the dose-dependent effect of delayed atomoxetine or fluoxetine therapy(starting on post-stroke day 5), coupled with limited physical exercise(2 hours daily voluntary wheel running;post-stroke days 9 to 42), on motor recovery of adult male mice after photothrombotic stroke.These drugs are selective norepinephrine or serotonin reuptake inhibitors indicated for disorders unrelated to stroke.The predetermined primary end-point for this study was motor function measured in two tasks of spontaneous motor behaviors in grid-walking and cylinder tests.Additionally, we quantified the running distance and speed throughout the study, the number of parvalbumin-positive neurons in the medial agranular cortex and infarct volumes.Both sensorimotor tests revealed that neither limited physical exercise nor a drug treatment alone significantly facilitated motor recovery in mice after stroke.However, combination of physical exercise with either of the drugs promoted restoration of motor function by day 42 post-stroke, with atomoxetine being a more potent drug.This was accompanied by a significant decrease in parvalbumin-positive inhibitory interneurons in the ipsilateral medial agranular cortex of mice with recovering motor function, while infarct volumes were comparable among experimental groups.If further validated in larger studies, our observations suggest that add-on atomoxetine or fluoxetine therapy coupled with limited, structured physical rehabilitation could offer therapeutic modality for stroke survivors who have difficulty to engage in early, high-intensity physiotherapy.Furthermore, in light of the recently completed Assessment o F Fluoxet INe In s Troke recover Y(AFFINITY) and Efficacy o F Fluoxetine-a randomis Ed Controlled Trial in Stroke(EFFECTS) trials, our observations call for newly designed studies where fluoxetine or atomoxetine pharmacotherapy is evaluated in combination w

关 键 词：drug repurposing neural repair physical exercise PHYSIOTHERAPY post-stroke recovery pre-clinical trial FLAME trial selective norepinephrine reuptake inhibitor selective serotonin reuptake inhibitor stroke pharmacotherapy 

分 类 号：R453[医药卫生—治疗学] R364[医药卫生—临床医学]