汞中毒大鼠QRS时限与Cx43及其Ser368位点磷酸化表达水平研究  

Study on abnormal QRS duration and expression of Cx43 and its phosphorylation level at Ser368 in mercury poisoning rats

作　　者：阎波[1] 杜艳秋[1] 邸晓莹 邹有硕[1] 张洪明[1] YAN Bo;DU Yan-qiu;DI Xiao-ying;ZOU You-suo;ZHANG Hong-ming(Shenyang Municipal Ninth People's Hospital,Shenyang 110024,China)

机构地区：[1]沈阳市第九人民医院,辽宁沈阳110024

出　　处：《中国工业医学杂志》2020年第5期400-402,420,F0003,共5页Chinese Journal of Industrial Medicine

基　　金：沈阳市科学技术局计划项目(编号:18-014-4-95)。

摘　　要：目的探讨汞中毒大鼠心电图QRS时限与心肌缝隙连接蛋白43(Cx43)及其Ser368位点磷酸化(pS368Cx43)表达水平。方法 40只Wistar大鼠随机分为对照组(0.2 ml生理盐水)及氯化汞低、中、高剂量染毒组(33.5、67.0、134.0μg/kg氯化汞溶液),皮下注射染毒,1次/d。染毒90 d后采集大鼠心电图,分析QRS时限改变,采用免疫组织化学染色法和Western blot法检测心室肌组织Cx43、pS368Cx43蛋白的表达情况;分析汞中毒大鼠QRS时限与Cx43及pS368Cx43表达量与分布改变的关系。结果与对照组相比,慢性汞中毒大鼠心电图QRS时限延长(P<0.05),心室肌组织Cx43、pS368Cx43表达量降低(P<0.05);Cx43和pS368Cx43在对照组主要分布于闰盘处,汞中毒组分布紊乱,部分呈现侧膜化改变。结论汞中毒大鼠QRS时限延长,其机制可能与汞中毒导致心肌Cx43和pS368Cx43表达降低及分布异常有关。Objective To investigate the correlation between the abnormal QRS duration and the expression of connexin 43(Cx43)and its phosphorylation level at Ser368(pS368Cx43)in mercury poisoning rats.Methods 40 Wistar rats were randomly divided into control group,low,middle and high dose mercury exposed groups,the exposed rats were subcutaneously injected with 33.5,67.0 and 134.0 μg/kg mercury chloride once a day,respectively.After 90 days of exposure,QRS duration was measured with electrocardiograms,the expression of Cx43 and pS368Cx43 in ventricular tissue were measured as well by immunohistochemical staining and Western blot,then the relationship between the expression of Cx43 and pS368Cx43 and the abnormal QRS duration was analyzed.Results The results showed that compared with the control rats,the QRS duration of mercury poisoning rats was obviously prolonged(P<0.05),meanwhile,the expressions of Cx43 and pS368Cx43 were also down-regulated(P<0.05).The distribution of Cx43 and pS368Cx43 in control rats were predominantly situated at the intercalated discs of ventricular myocardium,while that in mercury poisoning rats were more disordered,some of them showed lateral membrane changes.Conclusion The results suggested that mercury poisoning might prolong QRS duration,the molecular mechanism probably related to the decreased expression and abnormal distribution of Cx43 and pS368Cx43.

关 键 词：汞中毒 QRS时限 缝隙连接蛋白43(Cx43) 磷酸化 Ser368位点 

分 类 号：R135.13[医药卫生—劳动卫生] R994.3[医药卫生—公共卫生与预防医学]