植物病原丝状真菌寄生性与RGS蛋白的关系研究  

Relationship between parasitism and RGS protein in plant pathogenic filamentous fungi

作　　者：祝友朋 韩长志[1] ZHU Youpeng;HAN Changzhi(The Key Laboratory of Forest Disaster Warning and Control of Yunnan Province/College of Biodiversity Conservation,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学生物多样性保护学院/云南省森林灾害预警与控制重点实验室,昆明650224

出　　处：《华中农业大学学报》2020年第6期23-29,共7页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31960314);云南省应用基础研究计划项目(2018FG001-028)。

摘　　要：以49个全基因组序列已经公布的真菌为研究对象,通过OrthoVenn2同源基因簇比对、BLASTp比对和关键词搜索3种方法对G蛋白信号调控因子(regulators of G-protein signaling,RGS)同源基因进行比对分析,并结合SMART进行保守结构域分析,结果发现,49个真菌中共有229个RGS蛋白,每种真菌中所含有RGS蛋白的数量范围为3~9个,且死体营养型病原菌和半活体营养型病原菌的RGS蛋白数量高于活体营养型病原菌;根据RGS蛋白中保守结构域进行分类,可以划分为DEP-RGS、RGS-TM、PXA-RGS-PX、RGS、RGS-PAS-PAC、TM-RGS等6种,其中,具有RGS-PAS-PAC和TM-RGS这2种特殊保守结构域的RGS蛋白主要集中在半活体营养型病原菌和死体营养型病原菌;进一步对229个RGS蛋白进行遗传关系分析,发现具有同种保守结构域的RGS蛋白亲缘关系较近。上述研究结果表明植物病原丝状真菌的寄生性与RGS蛋白数量和种类之间存在着一定的关联性。Using 49 filamentous fungi with whole genome sequence published as the research objects,OrthoVenn2 homologous gene cluster alignment,BLASTp comparison,keyword search and SMART were used to analyze the homologous gene and conservative domain of regulators of G-protein signaling(RGS).The results showed that there were 229 RGS in the 49 fungi,the number of RGS in each fungus ranged from 3 to 9,and the number of RGS of the necrotrophs and hemibiotrophs fungi was higher than that of the biotrophs fungi.According to the conserve domains,the RGS proteins could be divided into six types,including DEP-RGS,RGS-TM,PXA-RGS-PX,RGS,RGS-PAS-PAC,and TM-RGS,of which RGS with two special conserved domains,RGS-PAS-PAC and TM-RGS,were mainly concentrated in the hemibiotrophs and necrotrophs fungi.Further genetic analysis showed that RGS proteins with the same conserved domain were closely related.The above results indicated that there was a certain correlation between the parasitism and the quantity and species of RGS protein in plant pathogenic filamentous fungi.

关 键 词：植物病原菌 丝状真菌 调控蛋白 G蛋白信号调控因子(RGS) 同源比对 比较基因组学 

分 类 号：S435.1[农业科学—农业昆虫与害虫防治]