GSH对铅胁迫下多年生黑麦草幼苗抗氧化系统的调控  

Effects of Glutathione(GSH)on Antioxidant Defense System of Lolium Perenne Seedlings under Lead Stress

作　　者：赵利清[1,2] 彭向永[3] 刘俊祥[1] 毛金梅[4] 孙振元[1] ZHAO Li-qing;PENG Xiang-yong;LIU Jun-xiang;MAO Jin-mei;SUN Zhen-yuan(Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Foresstry, Beijing 100091, China;The Open University of China, Beijing 100039, China;Qufu Normal University, Qufu, Shandong Province 273165, China;Economic Forest Research Institute Xinjiang Academy of Forestry, Urumqi, Xinjiang 830063, China)

机构地区：[1]国家林业和草原局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091 [2]国家开放大学,北京100039 [3]曲阜师范大学生命科学学院,山东曲阜273165 [4]新疆林业科学院经济林研究所,新疆乌鲁木齐830063

出　　处：《草地学报》2020年第6期1527-1534,共8页Acta Agrestia Sinica

基　　金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2018ZB002)资助。

摘　　要：本试验以12周龄的多年生黑麦草(Lolium perenne‘cuttle’)幼苗为试验材料,用铅(Lead,Pb),Pb+还原型谷胱甘肽(Glutathione,GSH)和Pb+丁硫氨酸-亚砜亚胺(L-Buthionine-sulfoximine,BSO)处理1周,研究GSH对Pb胁迫下多年生黑麦草抗氧化系统的调控机理,寻求缓解植物Pb胁迫的有效措施。结果表明:Pb胁迫下,外源GSH能够显著提高多年生黑麦草Pb的吸收和转运,增加抗氧化酶超氧化物歧化酶(Superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GPX)和谷胱甘肽还原酶(Glutathione reductase,GR)活性,显著增加抗氧化剂GSH含量及GSH/氧化型谷胱甘肽(Oxidized glutathione,GSSG)比率,提高了植物的还原力。外源GSH也能够显著降低活性氧超氧阴离子(Superoxide anion,O·-2)和过氧化氢(Hydrogen peroxide,H2O2)的产生量、膜的相对透性(Relative electric conductivity,EC)和丙二醛(Malondialdehyde,MDA)含量,保持了细胞的稳定性,减轻了膜脂的过氧化程度。外源BSO作用基本上与GSH相反。相关分析表明:GSH可通过提高SOD和GPX等抗氧化酶的活性,促进GSSG和GSH的相互转化,降低活性氧产生量,提高植物抵抗逆境胁迫的能力。In this study,Lolium perenne seedlings(12 weeks old)were exposed to Pb,Pb+GSH and Pb+BSO treatment for one week to investigate effects of glutathione on antioxidant defense system of leaf and root.The results showed that Pb+GSH treatment increased Pb absorption and transport.It also increased antioxidase activity such as superoxide dismutase(SOD),glutathione peroxidase(GPX)and glutathione reduction(GR),antioxidant GSH content and GSH/GSSG ratio.It enhanced Seedlings’reducing power.On the contrary,the content of ROS(O·-2and H2O2),malondialdehyde content(MDA)and relative electric conductivity(EC)declined under Pb+GSH treatment.Pb+BSO treatment had almost opposite effects to that of Pb+GSH treatment.The correlation analysis indicated that GSH could reduce reactive oxygen species production and alleviate oxidative stress by increasing antioxidase activity and promoting the mutual transformation of GSSG and GSH.

关 键 词：多年生黑麦草 谷胱甘肽 抗氧化系统 铅 

分 类 号：Q945.78[生物学—植物学] S812.8[农业科学—草业科学]