白羊草R2R3-MYB转录因子的挖掘及对干旱胁迫反应的研究  

Excavation of R2R3-MYB Transcription Factor and Its Response to Drought Stress in Bothriochloa ischaemum

作　　者：李春艳 王曦[1] 周胜花[1] 李希 钟华[2] 董宽虎[2] LI Chun-yan;WANG Xi;ZHOU Sheng-hua;LI Xi;ZHONG Hua;DONG Kuan-hu(Shanxi Agricultural University (Shanxi Academy of Agricultural Sciences), Taiyuan, Shanxi Province 030032, China;College of Agro-Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China)

机构地区：[1]山西农业大学山西省农业科学院,山西太原030032 [2]山西农业大学草业学院,山西太谷030801

出　　处：《草地学报》2020年第6期1784-1790,共7页Acta Agrestia Sinica

基　　金：山西省农业科学院博士基金项目(YBSJJ2008)资助。

摘　　要：本文以白羊草(Bothriochloa ischaemum)为材料,利用其目前已有的转录组序列,通过搜索、比对、功能域多态性分析、进化树构建和基因表达分析等手段,对白羊草R2R3-MYB型转录因子进行挖掘和干旱胁迫下的表达模式分析。结果从白羊草挖掘出43个R2R3-MYB转录因子,它们具有高度保守的[W]-X(19)-[W]-X(19)-[W]-X(n)-[W]-X(18)-[W]结构,主要参与生长发育、次生代谢和逆境响应等生物过程。通过进一步的分析,本研究筛选出8个可能参与干旱胁迫响应的R2R3-MYB转录因子。这为白羊草R2R3-MYB基因的功能研究和开发利用奠定了基础。This paper analyzed the expression pattern of the R2R3-MYB transcription factors of B.ischaemum under drought stress,through searching,comparison,functional domain polymorphism analysis,phylogenetic tree construction and gene expression analysis.A total of 43 R2R3-MYB transcription factors with highly conserved[W]-X(19)-[W]-X(19)-[W]-X(n)-[W]-X(18)-[W]were isolated from B.ischaemum.They were mainly involved in growth and development,secondary metabolism and stress responses.Through further analysis,there were eight R2R3-MYB transcription factors that may participate in drought stress response.This research lays a foundation for the functional analysis and application of R2R3-MYB.

关 键 词：白羊草 转录组数据库 干旱胁迫 R2R3-MYB转录因子 

分 类 号：Q945[生物学—植物学]