恰玛古乙醇提取物对博来霉素致肺纤维化大鼠的作用及其机制  

Effect of Brassica rapa L.ethanol extract on bleomycin-induced pulmonary fibrosis in rats and its mechanism

作　　者：袁志坚[1] 吴小瑜[1] 黄寅 何文涓 兰天 YUAN Zhi-jian;WU Xiao-yu;HUANG Yin;HE Wen-juan;LAN Tian(Department of Nursing,Wuxi Higher Health Vocational Technology School,Wuxi,Jiangsu 214028,China;Department of Pharmacology,China Pharmaceutical University,Nanjing,Jiangsu 211198,China)

机构地区：[1]无锡卫生高等职业技术学校护理系,江苏无锡214028 [2]中国药科大学药理学教研室,江苏南京211198

出　　处：《环境与职业医学》2020年第10期999-1004,共6页Journal of Environmental and Occupational Medicine

基　　金：江苏省无锡市卫生健康委员会医学科研项目(MS201658)。

摘　　要：[背景]现代社会环境问题日益严重,环境中粉尘、残留农药以及过敏原等均会导致肺纤维化,目前没有能有效缓解肺纤维化进展的方法和药物,中医药对肺纤维化的治疗作用日益受到重视。[目的]研究恰玛古乙醇提取液对博来霉素致肺纤维化大鼠的治疗作用,初步探究其对肺纤维化的作用机制。[方法]将50只SD大鼠随机分为对照组、模型组、地塞米松组(1mg·kg^-1,腹腔注射)和恰玛古不同剂量组(25、50 g·kg^-1,灌胃),除对照组外,其他各组动物均采用气管注射博来霉素(5mg·kg^-1)建立大鼠肺纤维化模型,建模7d后开始给药,连续给药21d。观察大鼠体重变化;用大鼠肺功能仪检测大鼠的用力肺活量(FVC)、第1秒用力呼气容积(FEV1)、呼气流量峰值(PEF);检测大鼠肺系数;采用苏木精-伊红染色检测肺组织形态学变化;用免疫组织化学法检测肺组织中Ⅰ型胶原和Ⅲ型胶原表达情况;用酶联免疫吸附试验检测肺组织中Ⅰ型胶原、Ⅲ型胶原和炎症因子肿瘤坏死因子(TNF)-α、白介素(IL)-6的含量。[结果]与模型组相比,第28天时:50g·kg^-1恰玛古组的大鼠体重明显增加[(262.7±30.2)g vs(235.4±38.9)g,P<0.05];25、50g·kg^-1恰玛古组的大鼠FEV1、FVC、PEF有不同程度升高[(6.15±0.28)、(6.81±0.36)mL vs(4.85±0.25)mL,(7.22±0.31)、(7.86±0.28)mL vs(5.92±0.23)mL,(30.61±8.88)、(32.14±6.27)mL vs(24.65±6.12)mL,P<0.05],肺系数减小[(0.58±0.09)%、(0.53±0.10)%vs(0.73±0.14)%,P<0.05],肺组织内炎性细胞浸润减少,肺泡壁断裂及肺泡腔融合较少,肺纤维化程度减轻;50g·kg^-1恰玛古组的大鼠肺组织内Ⅰ型胶原和Ⅲ型胶原表达降低[(2.15±0.56)mg·L^-1 vs(2.82±0.55)mg·L^-1,(2.35±0.58)mg·L^-1 vs(3.02±0.59)mg·L^-1,P<0.05];25、50 g·kg^-1恰玛古组的大鼠肺组织内炎症因子TNF-α、IL-6表达降低[(397.59±73.73)、(363.65±66.06)ng·L^-1 vs(554.59±115.81)ng·L^-1,(194.71±37.74)、(166.20±28.33)ng·L^-1 vs(253.39±7[Background]Environmental problems are increased dramatically in modern society.For example,dust,residual pesticides,and allergens can cause pulmonary fibrosis.At present,there are no effective methods and drugs to alleviate the progression of pulmonary fibrosis.Traditional Chinese medicine has received increasing attention for its potential therapeutic effect on the disease.[Objective]This experiment tests the therapeutic effect of Brassica rapa L.extract on pulmonary fibrosis induced by bleomycin,and explores relevant mechanism.[Methods]Fifty SD rats were randomly divided into a control group,a model group,a dexamethasone group(1mg·kg^-1,intraperitoneal injection),and two Brassica rapa L.groups(25 and 50 g·kg^-1,intragastrical administration).Except the control group,the other groups of rats were transtracheally injected with bleomycin(5mg·kg^-1)to establish a pulmonary fibrosis model.After 7 d,intraperitoneal injection of dexamethasone and intragastric administration of Brassica rapa L.extract lasted for 21 d.Body weight changes were observed.Rat lung forced vital capacity(FVC),forced expiratory volume in one second(FEV1),and peak expiratory flow(PEF)were measured with a rat pulmonary function meter.Lung coefficient was calculated.Histomorphological changes of lung were evaluated after HE staining.The expressions of collagenⅠand collagenⅢin lung tissues were detected after immunohistochemical staining.The contents of collagenⅠ,collagenⅢ,and inflammatory factors[tumor necrosis factor-α(TNF-α)and interleukin 6(IL-6)]in lung tissues were detected by enzymelinked immunosorbent assay.[Results]Compared with the model group,on day 28,the body weight of rats in the 50 g·kg^-1 Brassica rapa L.group was increased significantly[(262.7±30.2)g vs(235.4±38.9)g,P<0.05];the FVC,FEV1,and PEF of rats in the 25 and 50 g·kg^-1 Brassica rapa L.groups were increased[(6.15±0.28),(6.81±0.36)mL vs(4.85±0.25)mL;(7.22±0.31),(7.86±0.28)mL vs(5.92±0.23)mL;(30.61±8.88),(32.14±6.27)mL vs(24.65±6.12)mL,P<0.05],accom

关 键 词：恰玛古 肺纤维化 Ⅰ型胶原 Ⅲ型胶原 炎症因子 

分 类 号：R114[医药卫生—卫生毒理学]