同时测定人全血中铁、铜ICP-MS候选参考方法的建立  

A candidate reference method using ICP-MS for simultaneous determination of iron and copper in human blood

作　　者：蒲云罡 闫颖[2] 赵海舰[2] 曾洁[2] 周伟燕[2] 张天娇[2] 张传宝[2] Pu Yungang;Yan Ying;Zhao Haijian;Zeng Jie;Zhou Weiyan;Zhang Tianjiao;Zhang Chuanbao(National Center for Clinical Laboratories,Beijing Engineering Research Center of Laboratory Medicine,Beijing Hospital,National Center of Gerontology,Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100730,China;National Center for Clinical Laboratories,Beijing Engineering Research Center of Laboratory Medicine,Beijing Hospital,National Center of Gerontology,Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing 100730,China)

机构地区：[1]北京医院/国家老年医学中心/国家卫生健康委临床检验中心/北京市临床检验工程技术研究中心/中国医学科学院老年医学研究院/中国医学科学院北京协和医学院,北京100730 [2]北京医院/国家老年医学中心/国家卫生健康委临床检验中心/北京市临床检验工程技术研究中心/中国医学科学院老年医学研究院,北京100730

出　　处：《中华检验医学杂志》2020年第10期990-995,共6页Chinese Journal of Laboratory Medicine

基　　金：北京协和医学院2019年学科建设项目(201920102201)。

摘　　要：目的建立一种基于ICP-MS技术的全血铁、铜同时检测的候选参考方法。方法验证性研究。选取钴(Co)作为内标元素,采用称量法配制标准溶液及全血标本,对全血标本进行电热板消解并稀释,通过ICP-MS在同位素分析模式下测量57Fe/59Co、63Cu/59Co同位素比值,采用包括法定量。通过测定EQA质控品和有证标准物质,验证本方法的精密度、加标回收率和正确度,并通过方法比对进一步验证方法的性能。结果本法全血铁的检出限为0.136 mg/kg,定量限为0.454 mg/kg。全血铜的检出限为0.008 mg/kg,定量限为0.027 mg/kg。本方法标准曲线浓度范围为铜:0.83~3.33 mg/kg,铁:167~667 mg/kg,该范围内线性良好(R2>0.99990),方法的精密度良好,总CV范围:铁:0.42%~0.68%;铜:0.14%~0.94%。方法加标回收率均在100%左右,其范围:铁均值(99.69%~100.07%);铜均值(99.26%~100.51%)。结论成功建立了一种基于ICP-MS技术的全血铁、铜同时测定的候选参考方法,该方法相比传统参考方法简便、快速,精密度与加标回收率良好。Objective To establish a candidate reference method for simultaneous determination of whole blood iron and copper based on ICP-MS technology.Methods Cobalt(Co)was chosen as the internal standard,and was added gravimetrically into the standard solution and the whole blood sample.The whole blood sample was digested by electric heating plate and diluted.The isotopic ratios of 57Fe/59Co and 63Cu/59Co were measured by ICP-MS in the isotopic analysis mode.The precision,standard recovery and accuracy of the method were verified by measuring EQA materials and certified standard materials,and the performance of the method was further evaluated by method comparison.Results The detection limit of iron is 0.136 mg/kg and the limit of quantification was 0.454 mg/kg in this method.The detection limit of copper in whole blood was 0.008 mg/kg and the limit of quantification was 0.027 mg/kg.The standard curve of copper ranges from 0.83-3.33 mg/kg,iron ranges from 167-667 mg/kg.The calibration curve of iron and copper was good linearity(R2>0.99990).The precision of the method did well.The total CV range was 0.42%-0.68%for iron and 0.14%-0.94%for copper.The spike recoveries were all around 100%,the range of iron:99.69%-100.07%;copper:99.26%-100.51%.The correlation between this method and the existing clinical mass spectrometry methods was good.Conclusions A candidate reference ICP-MS method for simultaneous determination of whole blood Fe and Cu was established.This is a simple and rapid method with good precision and spike recovery compared to the traditional one.

关 键 词：电感耦合等离子体质谱法 铁 铜 内标法 候选参考方法 

分 类 号：R446.11[医药卫生—诊断学]