不同方法在先天性心脏病室间隔缺损动物模型建立中的对比研究  

Comparative study of different methods in establishing animal models of ventricular septal defect

作　　者：张国明[1] 何丽芸[1] 马松峰[1] ZHANG Guo-ming;HE Li-yun;MA Song-feng(The First Pediatric Ward of the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院儿外一病区,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《中国心血管病研究》2020年第11期1026-1029,共4页Chinese Journal of Cardiovascular Research

基　　金：新疆维吾尔自治区自然科学基金联合基金(2016D01C336)。

摘　　要：目的获取建立简便、效果稳定且可重复性好的先天性心脏病室间隔缺损小鼠动物模型的方法.方法⑴VPA动物模型:①VPA剂量处理组:对D7孕鼠分别腹腔注射VPA 200 mg/kg、400 mg/kg、600 mg/kg和700 mg/kg;②VPA时间处理组:对D6、D7、D8和D9孕鼠均腹腔注射VPA 700 mg/kg;对照组:孕鼠腹腔注射0.9%NaCl.⑵Hcy动物模型:高剂量组(Hcy 200 mg·kg^-1·d^-1),低剂量组(Hcy 100 mg·kg^-1·d^-1);对照组腹腔注射0.9%NaCl;孕鼠妊娠第7天起每日腹腔注射1次,直至孕第17天、19天再注射各1次.⑶VAD动物模型:实验组小鼠AIN-76A配方配制饲料,对照组每公斤饲料加入4000 IUVA/kg,喂养2.5个月实验组VAD后,与正常饮食雄鼠交配.观察胎鼠心脏畸形发生率.结果三组动物模型胎鼠心脏畸形发生率实验组均明显高于对照组(P<0.05),以VPA第7天,700 mg/kg剂量孕鼠组心脏畸形发生率11.17%为最高.结论三组动物模型实验组均可致胎鼠心脏发育畸形,以VPA组孕第7天,700 mg/kg剂量胎鼠心脏畸形发生率最高.Objective To obtain a simple.stable and reproducible CHD animal model of ventricular septal defect established by using three different methods.Methods(1)VPA animal model:①VPA dose treatment group:D7 pregnant mice were injected intraperitoneally with VPA 200 mg/kg.400 mg/kg.600 mg/kg and 700 mg/kg.②VPA time treatment group:D6.D7,D8 and D9 pregnant mice were injeted with VPA 700 mg/kg intraperitoneally and the control group pregnant mice were injected intraperitoneally with 0.9%Nacl.(2)Hey animal model:high-dose group(Hcy 200 mg·kg^-1·d^-1).low dose group(Hcy 100 mg·kg^-1·d^-1)and the control group was replaced with sterile normal saline and the pregnant mice were injected intraperitoneally 1 time daily from the 7th day of pregnancy,until the 17th day of pregnancy and another injection on the 19th day.(3)VAD animal model:The mice in the experimental group were prepared with AIN-76A formula and the control group was fed with 4000 IUVA/kg of feed for 2.5 months.After the VAD status in the experimental group,the females of the experimental and control groups were mated with males of normal diet.The incidence of the fetal mice heart malformations was observed.Results The incidence of fetal rats cardiac malformation in the three groups of animal model was significantly higher than that in the control group(P<0.05).The incidence of cardiac malformation in pregnant mice in the VPA(D7700 mg/kg)dose group was the highest,at 11.17%.Conclusion All three experimental animal model can cause fetal mice heart development abnormalities.The incidence of cardiac malfomation in pregnant mice in the VPA(D7700 mg/kg)dose group is the highest.

关 键 词：先天性心脏病 动物模型 丙戊酸 高同型半胱氨酸血症 维生素A缺乏 

分 类 号：Q95-33[生物学—动物学] R541.1[医药卫生—心血管疾病]