秦巴硒菇提取物(FA-2-b-β)对慢性髓系白血病原代细胞凋亡的影响  

Study of Qinba Mushroom Extract(FA-2-b-β)on Apoptosis of Chronic Myeloid Leukemia Primary Bone Marrow Cell

作　　者：程明霞 黄秀娟[1] 刘世栋 张文辉 韩彩娟 金大成 王东萍[1] 孙延庆[1,2] CHENG Mingxia;HUANG Xiujuan;LIU Shidong;ZHANG Wenhui;HAN Caijuan;JIN Dacheng;WANG Dongping;SUN Yanqing(Department of Hematology,Gansu Provincial People’s Hospital,Lanzhou 730000,China;Gansu University of Chinese Medicine,Lanzhou 730000,China;Department of Interventional Radiology,The First Hospital of Lanzhou University,Lanzhou 730000,China)

机构地区：[1]甘肃省人民医院血液内科,兰州730000 [2]甘肃中医药大学,兰州730000 [3]兰州大学第一附属医院心外科,兰州730000

出　　处：《中国现代应用药学》2020年第20期2433-2437,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(81560670);甘肃省自然科学基金项目(1606RJZA089);甘肃省中医药科学技术研究课题(GZK-2011-71);甘肃省中医药管理局科研课题(GZK-2013-8)。

摘　　要：目的探讨中药秦巴硒菇提取物(酸性RNA蛋白复合物FA-2-b-β)对人慢性髓系白血病(chronic myeloid leukemia,CML)原代细胞系凋亡的影响。方法采用CCK8法检测不同时间段(24,48,72 h)FA-2-b-β作用于CML原代细胞的增殖抑制率;流式细胞术检测细胞凋亡率和周期;蛋白印迹法测定相关凋亡蛋白Bcl-2、Bax、MET、CD44和β-catenin的表达。结果FA-2-b-β抑制CML原代细胞增殖,并呈时间、浓度依赖性。FA-2-b-β处理后,CML原代细胞凋亡率明显升高,促凋亡蛋白Bax表达升高,抗凋亡蛋白Bcl-2、MET、CD44和β-catenin表达降低。结论秦巴硒菇提取物通过下调凋亡相关蛋白抑制CML原代细胞增殖并诱导细胞凋亡,表现出较强的体外抗白血病作用。OBJECTIVE To investigate the effect of acidic RNA protein complex(FA-2-b-β)extracted from Qinba mushroom on apoptosis of primary human chronic myeloid leukemia(CML)bone marrow cell line.METHODS The cell proliferation rate was measured by CCK8 method at different time(24,48,72 h)points.Flow cytometry was used to detect cell apoptotic rate and cell cycle.Western blotting was used to detect the expression of Bcl-2,Bax,MET,CD44 andβ-catenin protein.RESULTS FA-2-b-βinhibited the proliferation of primary CML bone marrow cell line effectively in a dose and time dependent manner.After treated with Qinba mushroom,the apoptosis rates of primary CML bone marrow cell line increased,the expression of Bax increased,and the expression of Bcl-2,MET,CD44,β-catenin decreased.CONCLUSION Qinba mushroom extract inhibits proliferation,induces apoptosis of primary CML bone marrow cell line by regulating the related apoptotic protein.Qinba mushroom has strong anti-leukemia effect in vitro.

关 键 词：秦巴硒菇 慢性髓系白血病 原代白血病细胞 细胞凋亡 细胞周期 

分 类 号：R965.1[医药卫生—药理学]