大别山黄杜鹃根茎叶花内参基因的表达稳定性评价  

Evaluation of Suitable Reference Genes for qRT-PCR Normalizaiton in Rhododendron molle G.Don(Dabie Mountains)

作　　者：张羽佳 舒读 董雪 杜睿琛 李志良[1] 王书珍[1] Zhang Yujia;Shu Du;Dong Xue;Du Ruichen;Li Zhiliang;Wang Shuzhen(Hubei Collaborative Innovarion Center for the Characteristic Resources Exploitation of Dabie Mountains,College of Life Science,Huanggang Normal University,Hubei Zhongke Research Istitute of Industrial Technology,Huangung 438000)

机构地区：[1]黄冈师范学院生命科学学院,大别山特色资源开发湖北省协同创新中心,湖北省中科产业技术研究院,黄冈438000

出　　处：《分子植物育种》2020年第21期6963-6968,共6页Molecular Plant Breeding

基　　金：国家自然科学基金(31500995);湖北省知识产权局(2019-1-35)共同资助。

摘　　要：黄杜鹃(Rhododendron molle G. Don)是中国野生杜鹃花资源中极具观赏价值和药用价值的特有物种,其野生资源在大别山区零散分布,因此对其稳定内参基因的评估和筛选对黄杜鹃分子生物学和遗传学的研究至关重要。本研究以黄杜鹃根、茎、叶、花为试验材料,采用实时荧光定量PCR技术,分析RmRPL3-1、RmNAC-1、RmCOX1-4、RmPDS-1、RmUBQ-6、RmL10-4等候选内参基因在不同器官组织中的表达稳定性情况,并基于GeNorm、Normfinder和Best-Keeper软件分析荧光定量PCR的结果,筛选最优的内参基因。结果表明黄杜鹃不同组织中最稳定表达的内参基因为RmCOX1-4和RmUBQ-6的组合。本研究筛选的最稳定的内参基因将为黄杜鹃后续的基因表达研究提供校准依据。Rhododendron molle G. Don is the unique ornamental and medicinal value of wild rhododendron in China species, whose wild resources were scattered in Dabie mountains, were therefore evaluated for their stable internal reference genes and screened for molecular biology and genetics of rhododendron research is vital. In this study, expression pattern of candidate genes RmRPL3-1, RmNAC-1, RmCOX1-4, RmPDS-1, RmUBQ-6, and RmL10-4 were analyzed in different tissues of R. molle by real-time quantitative RT-PCR, including root, stem,leaf, and flower. According to Ge Norm, NormFinder, and Best-keepersoftwares, the most stable internal reference in different tissues of R. molle is the combination of RmCOX1-4 and RmUBQ-6. The most stable internal reference genes selected in this study will provide a basis for the following gene expression study in R. molle.

关 键 词：黄杜鹃(Rhododendron molle G.Don) 内参基因 实时荧光定量 表达谱 

分 类 号：S685.21[农业科学—观赏园艺]