芒果MCT基因的鉴定、进化及表达  

Gene Identification,Evolution and Expression of MCT Gene in Mango(Mangifera indica)

作　　者：盖江涛[1] 王鹏[1] 叶秀旭 陈业渊[1] Gai Jiangtao;Wang Peng;Ye Xiuxu;Chen Yeyuan(Topical Crops Genctic Resoures Istiule,Chinese Academy of Tropial Agricaulural Sciences,Haikou,571101)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海口571101

出　　处：《分子植物育种》2020年第21期6969-6974,共6页Molecular Plant Breeding

基　　金：海南省自然科学基金青年基金项目(319QN310);中国热带农业科学院基本科研业务费专项资金(1630032-019033)共同资助。

摘　　要：2-C-甲基-D-赤藓醇-4-磷酸胱氨酰转移酶(MCT)是MEP代谢途径中关键的酶。为了鉴定芒果中的MCT基因,探索MCT基因的进化路线和表达模式,本研究以拟南芥MCT基因为参考,利用BLAST和HMMER软件,从12个植物基因组中搜索得到了14个MCT基因,对其进行了保守结构域、系统发育分析,并对芒果中MCT基因的表达进行了分析。保守结构域分析结果显示,芒果MCT蛋白包含一个PF01128.19结构域,且蛋白序列高度保守。系统发育分析表明,MCT基因的进化与其所在物种的进化路径保持一致,藻类到被子植物中MCT基因的拷贝数受到严格控制。表达分析结果显示,在芒果品种‘桂热82’和‘红玉’中,MCT基因在发育过程中的芒果果皮、果肉中的表达量高于成熟芒果中果皮、果肉的表达量,推测其在芒果发育过程中有更重要的作用。本研究为进一步阐明芒果MCT基因的功能提供了科学依据。2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase(MCT) is a key enzyme involved in the 2-C-methyl-D-erythritol-4-phosphate( MEP) pathway. In order to identify the MCT gene in mango, study the evolutionary route and expression pattern of MCT gene. In this study, with arabidopsis MCT gene as a reference, 14 MCT genes were searched from 12 plant genomes through the BLAST and HMMER. Then, we analyzed the conserved domains, phylogeny, and the expression of MCT gene in mango. The results showed that the MCT protein of mango contains a PF01128.19 domain, and the protein sequences of MCT gene is highly conserved.Phylogenetic analysis showed that the evolution of MCT genes were consistent with the evolutionary route of its species, the number of copies of the MCT genes was tightly controlled from algae to angiosperms. The results of expression analysis showed that the expression of MCT gene in the peels and flesh of developing mango were higher than that in the peels and flesh of mature mango in ’Guire-82’ and ’Hongyu’. It is speculated that MCT gene play a more important role in the development of mango. These provide scientific basis for further elucidating the function of MCT gene in mango.

关 键 词：芒果(Mangifera indica) 2-C-甲基-D-赤藓醇-4-磷酸胱氨酰转移酶(MCT) 2-甲基-D-赤藓醇-4-磷酸(MEP) 

分 类 号：S667.7[农业科学—果树学]