粗山羊草响应盐胁迫转录组分析  

Transcriptome Analysis of Aegilops tauschii in Response to Salt Stress

作　　者：施田野 顾宇蓝 张磊 尹华燕 唐恒 平艳飞 穆如宇 穆可卿 张文文 侯文倩 Shi Tianye;Gu Yulan;Zhang Lei;Yin Huayan;Tang Heng;Ping Yanfei;Mu Ruyu;Mu Keqing;Zhang Wenwen;Hou Wenqian(School of Life Scicneces,Jangsu Normal Universiy,Xuzhou,21116;Collge of Agronomy,Shandong Agriculural University,Taian,271018;School of Biological and Agicultural Enginering,Weifang College,Weifang,261061)

机构地区：[1]江苏师范大学生命科学学院,徐州221116 [2]山东农业大学农学院,泰安271018 [3]潍坊学院生物与农业工程学院,潍坊261061

出　　处：《分子植物育种》2020年第21期7015-7022,共8页Molecular Plant Breeding

基　　金：江苏师范大学自然科学研究基金研究项目(18XLRX031,18XLRX030)资助。

摘　　要：利用转录组测序(RNA-Seq)技术对粗山羊草‘AL8/78'在200 mmol/L NaCl人工模拟盐胁迫0h和96h进行了转录组分析。结果表明:盐胁迫处理下上调和下调表达的差异表达基因(DEGs)分别为546个和876个;GO富集分析发现,DEGs主要集中在代谢过程、细胞过程、细胞连接、催化活性等生物过程;KEGG富集分析发现,DEGs主要富集在苯丙酮生物合成、类黄酮生物合成、植物激素信号转导等信号通路。通过对DEGs进行转录因子注释,共注释到41个转录因子,主要包括WRKY、MYB、bHLH、NAC和HSP等类型。利用实时荧光定量PCR(qRT-PCR)对DEGs进行了表达模式验证,发现其与RNA-Seq测序结果一致,证明了RNA-Seq结果的准确性。本研究为挖掘粗山羊草耐盐基因提供了理论基础。Transcriptomic analysis of crude goatgrass ’AL8/78’ was performed using RNA-Seq technology in200 mmol/L NaCl, which simulated salt stress for 0 h and 96 h artificially. The results showed that 546 genes were up-regulated and 876 genes were down-regulated under salt stress. GO enrichment analysis showed that DEGs were mainly involved in biological processes like metabolic process, cellular process, cellular junction and catalytic activity. KEGG enrichment analysis showed that DEGs were mainly involved in pathways such as phenylalanine metabolism, flavonoid biosynthesis and plant hormone signal transduction. 41 transcription factors were annotated in all DEGs including WRKY, MYB, bHLH, NAC and HSP. The expression pattern of DEGs were identified by qRT-PCR, which demonstrated the accuracy of RNA-Seq as the change of DEGs were the same between RNA-Seq and q RT-PCR. This research will provide theoretical basis for gene identification of Aegilops tauschii resistance to salt stress.

关 键 词：粗山羊草(Aegilops tauschii) 盐胁迫 转录组 

分 类 号：S567.2[农业科学—中草药栽培]