红掌花药培养及单倍体植株的鉴定  

Anther Culture and Haploid Identification of Anthurium andraeanum

作　　者：田丹青 葛亚英 潘晓韵 金亮 周媛 朱强 万晓 Tian Danqing;Ge Yaying;Pan Xiaoyun;Jin Liang;Zhou Yuan;Zhu Qiang;Wan Xiao(Zhejiang Institute of Landscape Plants and Flowers,Hangzhou,311251)

机构地区：[1]浙江省园林植物与花卉研究所,杭州311251

出　　处：《分子植物育种》2020年第21期7149-7154,共6页Molecular Plant Breeding

基　　金：浙江省‘十三五'林木新品种选育重大科技专项子项(2016C02056-13);杭州市科委种子种苗项目(2009103-2H15)共同资助。

摘　　要：以处于单核期的红掌花药为材料,经愈伤组织途径成功获得红掌单倍体新材料。通过对红掌花药培养、再生植株鉴定的研究表明:6℃1 d低温预处理显著降低了花药培养的污染率,提高了无菌花药的获得率和愈伤诱导率;花药愈伤诱导的适宜培养基为1/2 N6+2 mg/L 2.4-D+0.5 mg/L 6-BA+7%蔗糖+2%葡萄糖+6 g/L琼脂,增加蔗糖浓度有利于花药膨大;经根尖染色体压片法和流式细胞术法检测均得到了单倍体植株,但流式细胞术法更快速准确。实验结果可为红掌单倍体育种技术的深入研究提供材料支持。Haploid plantlet and callus were induced from the anther culture of Anthurium andraeanum as the explant at the stag of later uninucleate period. Further studies were carried on with another culture and regeneration plant identification. Results as below: Under 6℃ 1 d treatment, the pollution rate of another culture was decreased, and meanwhile, the rate of obtaining anthers and callus were improved;the best culture medium of callus induction was 1/2 N6+2 mg/L 2.4-D+0.5 mg/L 6-BA+7% sucros+2% glucose+6 g/L agar. Increasing the concentration of sucrose was conducive to anther expansion;haplophyte could be acquired in both root tip squash method and flow cytometry method, but the latter method was more accurate. The results of this experiment will provide materials and theoretical basis for further study.

关 键 词：红掌(Anthurium andraeanum) 花药培养 单倍体 倍性鉴定 

分 类 号：S682.14[农业科学—观赏园艺]