基于ISSR分子标记的皱皮木瓜遗传多样性分析  

Genetic Diversity Analysis of Chaenomeles speciosa(Sweet)Nakai Based on ISSR Molecular Markers

作　　者：蒋小刚 林先明 张美德[1,2] 王华 郭坤元[1,2] Jiang Xiaogang;Lin Xianming;Zhang Meide;Wang Hua;Guo Kunyuan(National Tnaditional Chinese Medicine Industry Technology System Enshi Comprehensive Test Starion,Institute of Chinese Medicinal Materials,Hubei Acadeny of Agricultural Sciences,Enshi,445000;Hubei Agricultural Science and Technology Innovation Center Chinese Medicinal Materials Sub-Center,Enshi,445000)

机构地区：[1]湖北省农业科学院中药材研究所,国家中药材产业技术体系恩施综合试验站,恩施445000 [2]湖北省农业科技创新中心中药材分中心,恩施445000

出　　处：《分子植物育种》2020年第21期7239-7245,共7页Molecular Plant Breeding

基　　金：国家现代农业产业技术体系建设专项(CARS-21);第三批“湖北省青年英才开发计划”项目(鄂青联发(2020)1号);湖北省技术创新专项(鄂西民族专项2019AKB092);恩施州科技计划研究与开发项目(D20180016)共同资助。

摘　　要：为了研究不同产地皱皮木瓜遗传多样性和亲缘性关系,本研究利用10个ISSR多态性引物对12份全国皱皮木瓜主产区的木瓜种质资源进行多态性和聚类分析,结果显示,各引物多态性百分率范围为65%~100%,皱皮木瓜种质资源的遗传相似系数在0.5057-0.8621之间,各种质间遗传信息较为丰富。聚类分析将12份种质划分为3个组,第1组又分为2个亚组,其中M4(湖南省桑植县-1)和M7(湖南省桑植县-2)亲缘关系最近,M1(云南省洱源县)和M10(湖北省郧西县)亲缘关系最远。以上结果表明12份全国皱皮木瓜主产区的木瓜种质资源具有较高的遗传多样性。In order to study the genetic diversity and genetic relationship of Chaenomeles speciosa(Sweet)Nakai from different producing areas,10 ISSR polymorphic primers were used in the polymorphism and cluster analysis of 12 Chaenomeles speciosa(Sweet)Nakai germplasm resources in the main producing areas.The results showed that the percentage of polymorphism of each primer ranged from 65%to 100%,and the genetic similarity coefficient of Chaenomeles speciosa(Sweet)Nakai germplasm resources ranged from 0.5057 to 0.8621.The genetic information of various interstitials was abundant.Cluster analysis divided 12 accessions into 3 groups,and the first group was divided into 2 subgroups.Among them,the relationship of M4(Sangzhi County-1,Hunan Province)and M7(Sangzhi County-2,Hunan Province)was closest,the relationship of M1(Eryuan County,Yunnan Province)and M10(Yunxi County,Hubei Province)was farthest.These results indicate that there is a high genetic diversity of 12 Chaenomeles speciosa(Sweet)Nakai germplasm resources in the main producing areas in China.

关 键 词：皱皮木瓜(Chaenomeles speciosa(Sweet)Nakai) ISSR分子标记 遗传多样性 亲缘关系 

分 类 号：S661.6[农业科学—果树学]