临床样本A亚型人呼吸道合胞病毒二代测序全基因组特征分析  

Whole-genome sequence analysis of human respiratory syncytial virus subgroup A from second-generation sequencing of clinical samples

作　　者：郭琼 王超[1] 黄益曼 张骞[1] 王浩 麻粉莲[1] 郑丽舒[1] Guo Qiong;Wang Chao;Huang Yiman;Zhang Qian;Wang Hao;Ma Fenlian;Zheng Lishu(NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing100052,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,国家卫生健康委员会医学病毒与病毒病重点实验室,北京100052

出　　处：《中华实验和临床病毒学杂志》2020年第5期478-484,共7页Chinese Journal of Experimental and Clinical Virology

基　　金："艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(2018ZX10102001);侯云德院士科研青年基金项目(2019HYDQNJJ06)。

摘　　要：目的分析北京市一株人呼吸道合胞病毒(human respiratory syncytial virus,HRSV)A亚型全基因组序列的变异和遗传特征。方法对北京友谊医院住院儿童鼻咽抽吸物样本核酸进行二代测序,获得了一株HRSV A亚型全基因组序列,与其他相关参考株构建系统发生树,并对主要蛋白编码区进行同源性分析、单核苷酸多态性分析以及N-糖基化位点预测。结果系统发生树和同源性分析结果提示,本研究获得的HRSV病毒株(RSVA/Beijing-China/2017)属于A亚型ON1基因型。核苷酸和氨基酸变异分析表明G蛋白、F蛋白和L蛋白变异性较大。氨基酸变异位点分析显示G蛋白第142位发生了氨基酸替换(L142S)。F蛋白P27肽中(110-136aa)有一处氨基酸替换(S105N),在抗原位点Ø(62-69aa和196-210aa)中也发生了氨基酸替换(C69Y)。N-糖基化位点预测发现该毒株的F蛋白N-糖基化位点有5个,G蛋白N-糖基化位点有4个。结论本研究所得的HRSV毒株属于A亚型ON1基因型,G蛋白、F蛋白和L蛋白变异较大,G和F蛋白共发生22处氨基酸替换。Objective To study the sequence characteristics and genetic variation of a human respiratory syncytial virus(HRSV)subtype A genome in Beijing.Methods The genomic RNA of HRSV from nasopharyngeal aspirate samples was sequenced and obtained a whole genome sequence of HRSV A subtype.The phylogenetic tree was constructed with reference sequences of other HRSV strains.The major proteins were compared and single nucleotide polymorphism analyzed.In addition,the N-glycosylationsites of F and G protein were predicted.Results Phylogenetic tree and homology analysis results suggest that the HRSV strain(RSVA/Beijing-China/2017)was the A subtype ON1 genotype.Nucleotide and amino acid variation analysis showed that G protein,F protein and L protein had some substitutions.Analysis of amino acid variation sites showed that amino acid substitution(L142S)occurred at position 142 of G protein.For F protein,there were two substitutions,which were S105N in the P27 peptide(110-136aa)and C69Y in the antigen sitesØ(62-69 aa and 196-210 aa).The prediction of N-glycosylation sites revealed that there were 5 N-glycosylation sites of F protein and 4 N-glycosylation sites of G protein in this strain.Conclusions The HRSV strain obtained in Beijing belongs to A subtype ON1 genotype.The G,F and L proteins have large variations,and 22 amino acid substitutions have occurred in the G and F proteins.

关 键 词：呼吸道感染 人呼吸道合胞病毒(HRSV) 全基因组 

分 类 号：R725.1[医药卫生—儿科] R440[医药卫生—临床医学]