敲低胆碱激酶α(CHKA)抑制U87MG人胶质瘤细胞增殖和侵袭及迁移  

Knockdown of choline kinaseα(CHKA)inhibits the proliferation,invasion and migration of human U87MG glioma cells

作　　者：岳芳倩 邹有瑞[2] 孙胜玉[2] 王哲 黄灵 马辉[2] YUE Fangqian;ZOU Yourui;SUN Shengyu;WANG Zhe;HUANG Ling;MA Hui(Clinical Medical College,Ningxia Medical University,Yinchuan 750004,China;Department of Neurosurgery,General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学临床医学院,宁夏银川750004 [2]宁夏医科大学总医院神经外科,宁夏银川750004

出　　处：《细胞与分子免疫学杂志》2020年第8期724-728,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81760449)。

摘　　要：目的检测胶质瘤组织胆碱激酶α(CHKA)的表达并进一步探讨敲低CHKA对U87MG人胶质瘤细胞增殖、侵袭和迁移能力的影响。方法采用实时定量PCR检测高级别胶质瘤组织和外伤脑组织CHKA的mRNA表达,Western blot法检测肿瘤组织和外伤组织CHKA蛋白表达。构建CHKA短发夹RNA(shCHKA)慢病毒及其对照慢病毒(shNC)并感染U87MG胶质瘤细胞,分为shCHKA组、载体对照组和空白对照组。CCK-8法检测U87MG细胞增殖,TranswellTM侵袭实验检测胶质瘤细胞侵袭能力,划痕愈合实验检测其迁移能力。结果胶质瘤组织中CHKA mRNA和蛋白高表达。敲低CHKA基因后,抑制U87MG胶质瘤细胞的增殖、侵袭和迁移。结论CHKA在胶质瘤组织中的表达量明显高于正常脑组织且下调CHKA可抑制胶质瘤细胞的增殖、侵袭和迁移能力,CHKA可能是一种促癌基因与胶质瘤的发生发展有关。Objective To detect the expression of choline kinaseα(CHKA)in glioma and to further explore the effect of CHKA knockdown on the proliferation,invasion and migration of U87MG human glioma cells.Methods The mRNA expression of CHKA in high-grade gliomas and traumatic brain tissues was detected by real-time quantitative PCR.The expression of CHKA protein in high-grade gliomas and traumatic brain tissues was detected by Western blot analysis.The short hairpin RNA of CHKA(shCHKA)lentivirus and its control lentivirus(shNC)were constructed and used to infect U87MG glioma cells,which were then divided into the following three groups:shCHKA group,shNC group and blank control group.The proliferation of U87MG cells was examined by CCK-8 assay,the invasion ability of glioma cells was tested by TranswellTM invasion assay,and the migration ability of glioma cells was evaluated by scratch healing test.Results The CHKA mRNA and protein were highly expressed in glioma.Knockdown of CHKA gene inhibited the proliferation,invasion and migration of U87MG glioma cells.Conclusion The expression of CHKA in glioma tissue is significantly higher than that in the normal brain tissue,and knockdown of CHKA gene inhibits the proliferation,invasion and migration of glioma cells.It suggests that CHKA may be related to the occurrence and development of gliomas.

关 键 词：胆碱激酶α(CHKA) U87MG细胞 细胞增殖 细胞侵袭 细胞迁移 

分 类 号：Q279[生物学—细胞生物学] R392-33[医药卫生—免疫学]