2019年天津市急性胃肠炎人群诺如病毒基因分型分析  

Molecular characteristics of norovirus in acute gastrointestinal illness,Tianjin,2019

作　　者：雷玥[1] 庄志超 田宏[1] 李晓燕[1] Lei Yue;Zhuang Zhichao;Tian Hong;Li Xiaoyan(Department of Pathogen Detection,Tianjin Centers for Disease Control and Prevention,Tianjin 300011,China)

机构地区：[1]天津市疾病预防控制中心病原生物检测室,天津300011

出　　处：《疾病监测》2020年第10期913-919,共7页Disease Surveillance

基　　金：国家科技重大专项(No.2017ZX10103007–002);天津市疾病预防控制中心科研专项基金(No.CDCKY1701)。

摘　　要：目的分析2019年天津市急性胃肠炎人群中诺如病毒的基因特征。方法本研究采集2019年全年天津市各区疾病预防控制中心送检的散发、暴发、聚集性腹泻诺如病毒阳性粪便标本,采用反转录聚合酶链式反应(RT-PCR)方法将标本进行扩增,对阳性产物进行聚合酶区和衣壳区序列测定,构建系统进化树。结果诺如病毒总体阳性率为6.01%(97/1615),除1月无样本检测外,其他各月份均有诺如病毒的检出。其中GⅠ组检出率1.11%(18/1615),GⅡ组检出率5.02%(81/1615)。聚合酶区和衣壳区基因分型结果:2019年的基因型别为GⅡ.2[P16]、GⅡ.4[P31]、GⅡ.17[P17]、GⅠ.3[P13]、GⅡ.6[P7]、GⅠ.5[P4]、GⅡ.3[P12]、GⅠ.5[P12]、GⅠ.1[P1]、GⅠ.2[P2]等。13起急性胃肠炎暴发或聚集性腹泻病例中占比前3位的基因重组株为GⅡ.2[P16](30.77%,4/13)、GⅡ.4[P31](23.08%,3/13)、GⅡ.17[P17](23.08%,3/13)。结论天津市诺如病毒感染的基因型呈多样性,不同基因型持续共同进化和循环,需进一步加强诺如病毒病原监测及分子分型鉴定工作。Objective To analyze the genetic characteristics of norovirus in acute gastrointestinal illness population in Tianjin in 2019.Methods Stool samples from acute gastroenteritis patients in outbreaks and clusters of the disease or sporadic cases collected by all 16 district CDCs in Tianjin were detected with RT-PCR.The polymerase and capsid genes of the isolated strains were amplified.Phylogenetic tree was constructed for sequences analysis.Results A total of 1615 samples were detected by district-level CDCs,in which 97 were positive for norovirus(6.01%).The positive samples were detected all the year around except January.The positive rate was 1.11%for norovirus GⅠand 5.02%for norovirus GⅡrespectively.The result of genotyping of both polymerase and capsid was as follow:GⅡ.2[P16],GⅡ.4[P31],GⅡ.17[P17],GⅠ.3[P13],GⅡ.6[P7],GⅠ.5[P4],GⅡ.3[P12],GⅠ.5[P12],GⅠ.1[P1]and GⅠ.2[P2].The top three genetic recombination strains of norovirus detected in 13 cases of acute gastroenteritis in outbreaks and clusters of the disease were GⅡ.2[P16](30.77%,4/13),GⅡ.4[P31](23.08%,3/13)and GⅡ.17[P17](23.08%,3/13).Conclusion The genotypes of norovirus was diverse in Tianjin.The coevolution and circulation of different genotypes continue,so it is necessary to further strengthen the surveillance and molecular typing of norovirus.

关 键 词：诺如病毒 基因型 重组 腹泻 急性胃肠炎 

分 类 号：R211[医药卫生—中医学] R511