三种实验室常规方法对流感病毒灭活效果评价  

Evaluation of three laboratory routine methods for inactivating influenza virus

作　　者：陈爱珺[1] 郑丽舒[1] 姚立红[1] 王超[1] 张骞[1] 郭建强[1] Chen Aijun;Zheng Lishu;Yao Lihong;Wang Chao;Zhang Qian;Guo Jianqiang(NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,China CDC,Beijing 100052,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,国家卫生健康委员会医学病毒与病毒病重点实验室,北京100052

出　　处：《中华实验和临床病毒学杂志》2020年第4期415-419,共5页Chinese Journal of Experimental and Clinical Virology

基　　金："艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(2018ZX10734401-018)。

摘　　要：目的针对3种实验室常规消毒方法对流感病毒灭活效果进行评价。方法采用紫外照射物理方法及乙醇和84消毒液2种化学方法,分别对流感病毒进行消杀,不同时间或浓度的消毒方法处理病毒样本后感染MDCK细胞,观察细胞病变效应(cytopathic effect,CPE)鉴定对流感病毒灭活效果。结果紫外照射流感病毒时间≥1 min、或75%乙醇处理流感病毒0.25~32 min、或84消毒液有效氯浓度≥400 mg/L且室温消杀流感病毒时间≥1 min,上述3种条件下均检测不到病毒感染性。结论摸索了实验室常规消毒方法灭活流感病毒的条件,为建立流感病毒实验室防护规范和应急处置技术方案提供了参考数据。Objective To evaluate the effectiveness of three laboratory routine disinfection methods on influenza virus inactivation.Methods The physical method of ultraviolet irradiation and two chemical methods of ethanol and"84 disinfectant"were used to inactivate the influenza virus respectively.After disinfecting the virus samples at different times or concentrations,MDCK cells were inoculated and the inactivation effect of influenza virus was identified by observing cytopathic effect(CPE).Results When the influenza virus was irradiated by ultraviolet light for≥1 min,treated with 75%ethanol for 0.25-32 min,the effective chlorine concentration of"84 disinfectant"was≥400 mg/L,and the influenza virus was disinfected at room temperature for≥1 min,no virus infectivity could be detected under the three conditions.Conclusions The condition of inactivating influenza virus by routine disinfection methods in laboratory was explored,which provided reference data for establishing the protection specifications and the emergency treatment plans for influenza virus laboratories.

关 键 词：紫外照射 消毒液 灭活 流感病毒 应急处置 

分 类 号：R373.13[医药卫生—病原生物学]