2016—2018年济南市生活污水轮状病毒A组分子流行病学特征分析  

Molecular epidemiological characterization of group A rotavirus in domestic sewage in Jinan from 2016 to 2018

作　　者：杜海波 林小娟[2] 刘姗姗 纪峰[2] 陶泽新[2] 宋艳艳 徐爱强 Du Haibo;Lin Xiaojuan;Liu Shanshan;Ji Feng;Tao Zexin;Song Yanyan;Xu Aiqiang(School of Public Health,Cheeloo College of Medicine,Shandong University,Jinan 250012,China;Institute of Immunization and Prevention,Shandong Center for Disease Control and Prevention,Jinan 250014,China;Organization and Personnel Section,Xiangyang Center for Disease Control and Prevention,Xiangyang 441000,China)

机构地区：[1]山东大学齐鲁医学院公共卫生学院,济南250012 [2]山东省疾病预防控制中心免疫预防管理所,济南250014 [3]襄阳市疾病预防控制中心组织人事科,441000

出　　处：《中华预防医学杂志》2020年第10期1115-1120,共6页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金项目(81573209);泰山学者工程专项经费资助(ts201511105)。

摘　　要：目的:了解生活污水中的A组轮状病毒(RVA)的检出情况及分子流行病学特征。方法:本研究于2016—2018年3年期间,在济南市每月采集生活污水,获得的36份污水样本通过阴离子膜吸附洗脱法浓缩后,进行核酸提取,RT-PCR扩增RVA VP7和VP4基因片段,再经纯化、TA克隆和测序后,对所获得的序列进行基因定型、同源性分析和系统发生学分析。结果:36份污水样本中有31份检测出RVA G基因(检出率为86.1%),33份样本检测出RVA P基因型(检出率为91.7%)。共获得RVA序列536条,其中G基因型序列225条,分属于6个基因型,G9型别占比最多为92.4%(208条);获得P基因型序列311条,分属于4个基因型,优势型P[8]占50.1%(156条),P[4]次之为41.8%(130条)。系统发生学分析显示优势型别G9、P[8]在当地均存在多个传播链共循环。结论:本研究通过对济南市生活污水所获得的RVA相关序列进行型别、同源性、系统发生学特征的分析,进一步证实RVA环境监测可行且必要。Objective To understand the detection of group A rotavirus(RVA)in domestic sewage and its molecular epidemiological characteristics,and further explore the feasibility and necessity of RVA environmental surveillance.Methods From 2016 to 2018,we collected domestic sewage samples monthly in Jinan city,and concentrated them via anion membrane adsorption-elution method.Then RNA extraction and RVA VP7 and VP4 coding region RT-PCR amplification were performed.After purification,TA cloning and sequencing,homology analysis and phylogenetic analysis were conducted on the obtained sequences.Results RVA G gene was detected in 31 of the 36 sewage samples(86.1%detection rate);RVA P genotype was detected in 33 samples(91.7%detection rate).A total of 536 RVA sequences were obtained,of which 225 G-type sequences belonged to 6 genotypes,and the G9 accounted for 92.4%(208/225);311 P-type sequences were obtained,which belonged to 4 genotypes.The dominant P[8]accounted for 50.1%(156/311),followed by P[4]with 41.8%(130/311).Phylogenetic analysis shows that there were multiple transmission chains circulating in the dominant genotypes G9 and P[8].Conclusion The genotype,homology,and phylogenetic characteristics of sequences obtained from domestic sewage in Jinan area were described,which further confirmeing that RVA environmental surveillance is not feasible but also necessary.

关 键 词：轮状病毒属 分子流行病学 环境监测 

分 类 号：R123.1[医药卫生—环境卫生学] R512.5[医药卫生—公共卫生与预防医学]