蜂胶中提取的咖啡酸苯乙酯和咖啡酸苄酯抑制食管癌Eca109细胞的功效  

The inhibitory effects of caffeic acid phenethyl ester and caffeic acid benzyl ester extracted from propolis on esophageal carcinoma Eca109 cells

作　　者：刘会 袁雯雯 李俊雅 玄红专[1] LIU Hui;YUAN Wen-wen;LI Jun-ya;XUAN Hong-zhuan(School of Life Science,Liaocheng University,Liaocheng 252059,China)

机构地区：[1]聊城大学生命科学学院,聊城252059

出　　处：《天然产物研究与开发》2020年第11期1904-1912,1927,共10页Natural Product Research and Development

基　　金：山东省现代农业产业技术体系蜂产业创新团队蜂产品加工与质量控制(SDAIT-24-05);农业部蜂产品质量安全控制重点实验室开放课题(FCPZLAQ-2018-03)。

摘　　要：本文研究了蜂胶中两种主要组分咖啡酸苯乙酯(CAPE)和咖啡酸苄酯(CABE)对食管癌Eca109细胞的抑制功效及初步作用机理。通过SRB法检测了细胞存活率;划痕法结合Transwell实验检测对Eca109细胞迁移和浸润的影响;DAPI染色结合Western blotting检测了对Eca109细胞凋亡和凋亡执行者Caspase 3和PARP蛋白表达的影响;通过两种荧光探针分别检测了对细胞内活性氧(ROS)和线粒体膜电位(MMP)的影响。结果表明,CAPE和CABE能够显著抑制Eca109细胞增殖,诱导其凋亡,上调Caspase 3和PARP的水平,同时CAPE和CABE显著上调ROS水平并降低MMP水平。说明CAPE和CABE是两种有潜力的治疗食管癌的天然产物。The inhibitory effects of caffeic acid phenethyl ester(CAPE)and caffeic acid benzyl ester(CABE)extracted from propolis on esophageal carcinoma Eca109 cells were studied in the present study.Cell viability was measured by SRB assay.Cell apoptosis was tested by DAPI staining and Western blotting.Tumor invasion and migration were studied by Transwell experiment and scratch assay.Reactive oxygen species(ROS)and mitochondrial membrane potential(MMP)were tested by two different fluorescence probes.The results showed that CAPE and CABE significantly inhibited the proliferation of Eca109 cells,and obviously suppressed migration and invasion of Eca109 cells.Moreover,CAPE and CABE induced apoptosis by up-regulating the levels of Caspase 3 and PARP,and increasing ROS levels and decreasing MMP levels.CAPE and CABE are two promising natural compounds in the treatment of esophageal carcinoma.

关 键 词：蜂胶 咖啡酸苯乙酯 咖啡酸苄酯 食管癌 凋亡 

分 类 号：S896.6[农业科学—特种经济动物饲养]