云南汉族CRHBP基因多态性与精神分裂症及其攻击行为的相关性分析  

Analysis of Correlation between Gene Polymorphisms of CRHBP in Yunnan Han Population and Schizophrenia and Aggressive Behavior

作　　者：陈阳 胡利平 刘林林 张秀峰 计艾岑 李佳珏 聂胜洁 CHEN Yang;HU Li-ping;LIU Lin-lin;ZHANG Xiu-feng;JI Ai-cen;LI Jia-jue;NIE Sheng-jie(Forensic Science College of Kunming Medical University,Kunming 650500,China;Dian Regional Fo-rensic Science Institute Hunan,Changsha 410006,China)

机构地区：[1]昆明医科大学法医学院,云南昆明650500 [2]湖南迪安司法鉴定中心,湖南长沙410006

出　　处：《法医学杂志》2020年第5期672-676,共5页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(31660310,31860300);云南省科技厅-昆明医科大学联合专项面上资助项目[2017FE467(-022)]。

摘　　要：目的探讨促肾上腺皮质激素释放激素结合蛋白基因4个标签单核苷酸多态性(tag single nucleo⁃tide polymorphism,Tag SNP)位点(rs7721799、rs32897、rs7718461、rs10062367)与云南汉族个体精神分裂症及患者攻击行为的相关性。方法采用病例对照关联分析,建立复合扩增体系,应用改良的多重高温连接酶检测反应技术检测163例汉族精神分裂症患者(其中伴攻击行为患者81例,不伴攻击行为患者82例)和345例健康汉族个体CRHBP基因4个SNP位点基因型,采用SPSS 19.0、Haploview 4.2、PHASE 2.1软件进行统计学分析。结果CRHBP基因rs7718461位点以外的3个SNP位点与精神分裂症的发病无关联(P>0.05)。rs7718461位点携带GG或GA基因型患者的攻击行为相对风险为携带AA基因型患者的4.903倍(P<0.05)。结论CRHBP基因可能与云南汉族精神分裂症的发生无关联,但rs7718461的AA基因型可能降低精神分裂症患者攻击行为发生的风险。Objective To explore the correlation between 4 tag single nucleotide polymorphisms(Tag SNP)sites(rs7721799,rs32897,rs7718461,rs10062367)of corticotropin releasing hormone binding protein(CRHBP)and schizophrenia and aggressive behavior in the Yunnan Han population.Methods Case-control correlation analysis was used to establish a complex amplification system.Improved multiplex ligase detection reaction(iMLDR)technology was used to detect the genotypes of 4 SNP sites of CRHBP gene of 163 Han schizophrenic patients(including 81 patients with aggressive behavior,82 patients without aggressive behavior)and 345 healthy Han individuals,which were analyzed statistically by SPSS 19.0,Haploview 4.2 and PHASE 2.1 software.Results There was no correlation between the 3 SNP sites of CRHBP gene and the onset of schizophrenia except for the rs7718461 site(P>0.05).The relative risk of aggressive behavior of patients carrying GG or GA genotype at rs7718461 site were 4.903 times higher than those carrying AA genotype(P<0.05).Conclusion The CRHBP gene may not be associated with the occurrence of schizophrenia in Yunnan Han population,but AA genotype of rs7718461 may reduce the risk of aggressive behavior in schizophrenia patients.

关 键 词：司法精神病学 法医遗传学 多态性 单核苷酸 精神分裂症 攻击 汉族 云南 

分 类 号：DF795.1[医药卫生—法医学]