香烟烟雾提取物对可溶性内皮细胞蛋白C受体表达的影响  

Effects of Cigarette Smoke Extract on the Expression of Protein C Receptor in Endothelial Cells

作　　者：樊芳芳[1] 胡晓芸[1] FAN Fangfang;HU Xiaoyun(First Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi,China)

机构地区：[1]山西医科大学第一医院,太原030001

出　　处：《中西医结合心脑血管病杂志》2020年第20期3362-3365,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

摘　　要：目的观察不同条件香烟烟雾提取物(CSE)作用于人脐静脉内皮细胞(HUVECs),观察CSE对可溶性内皮细胞蛋白C受体(sEPCR)表达的影响。方法选用第4代~第6代HUVECs,将其接种于培养板中,将0%、2.5%、5.0%及10.0%的CSE与HUVECs共同孵育,8 h后收集各浓度组上清液;将选出的5.0%CSE与HUVECs分别孵育0 h、4 h、8 h、12 h及24 h,分别收集各组上清液。将收集的上清液采用酶联免疫吸附法测定sEPCR表达变化。结果不同浓度CSE作用于HUVECs 8 h后,与0%CSE比较,5.0%、10.0%CSE组sEPCR表达升高,差异均有统计学意义(P<0.05)。5.0%CSE与HUVECs分别孵育不同时间,8 h、12 h及24 h sEPCR表达较对照组升高,差异均有统计学意义(P<0.05)。结论CSE可损伤血管内皮细胞,引起sEPCR升高,降低蛋白C/活化蛋白C活性,影响凝血功能,从而增加血栓发生率。Objective To investigate the effects of different cigarette smoke extract(CSE)on the expression of soluble endothelial cell protein C receptor(sEPCR)in human umbilical vein endothelial cells(HUVECs).Methods The 4th to 6th generation HUVECs were selected and inoculated into culture plates.0%,2.5%,5.0%and 10.0%CSE were incubated with HUVECs,and the supernatant of each group was collected after 8 hours.5.0%CSE and HUVECs were incubated for 0 h,4 h,8 h,12 h and 24 h respectively.The supernatant of each group was collected.The expression of sEPCR was detected by enzyme-linked immunosorbent double antibody sandwich assay.Results After incubation with different concentrations of CSE for 8 h,the expressions of sEPCR were significantly higher in 5.0%and 10.0%CSE groups than that in 0%CSE group(P<0.05).5.0%CSE and HUVECs were incubated for 0 h,4 h,8 h,12 h and 24 h,the expression of sEPCR in CSE group for 8 h,12 h and 24 h was higher than that in the control group at 8 h,12 h and 24 h(P<0.05).Conclusion CSE could damage vascular endothelial cells,increase sEPCR,reduce protein C/activated protein C activity,affect blood coagulation function,thereby increase the incidence thrombosis.

关 键 词：香烟烟雾提取物 可溶性内皮细胞蛋白C受体 人脐静脉内皮细胞 血栓形成 实验研究 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]