光源-探测器小距离散射光谱在疼痛测量中的应用  

Study on the Application of Scattering Spectrum With Small Source-Detector Separation in Pain Measurement

作　　者：戴丽娟[1] 丁乐明 李韪韬[2] 钱志余[2] DAI Li-juan;DING Le-ming;LI Wei-tao;QIAN Zhi-yu(Department of Mechanical Engineering,Nantong University,Nantong 226019,China;Department of Biomedical Engineering,Nanjing University of Aeronautics and Astronautics,Nanjing 210016,China)

机构地区：[1]南通大学机械工程学院,江苏南通226019 [2]南京航空航天大学生物医学工程系,江苏南京210016

出　　处：《光谱学与光谱分析》2020年第12期3743-3747,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(61405098,61875085);2017年江苏政府留学奖学金资助。

摘　　要：提出了利用光源-探测器小距离散射光谱进行疼痛测量的一种新方法。首先,基于修正的Beer-Lambert定律,建立氧合血红蛋白和脱氧血红蛋白浓度变化与光源-探测器小距离探头所采集的散射光谱强度变化的关系式,通过悬乳液血液混合液体模型实验,将公式计算结果与组织血氧仪测量结果进行对比。其次,分别对7只大鼠的后肢注射福尔马林溶液以制造疼痛刺激,用光源-探测器小距离光谱系统采集注射前、后大鼠前扣带回的散射光谱,同时,用生物电信号采集系统获取注射前、后大鼠前扣带回的局部放电信号。最后,计算氧合血红蛋白浓度、脱氧血红蛋白浓度和总血红蛋白浓度的变化,获取β波频段(13~30 Hz)每10 s的功率强度,分析这些参数在注射前、后的变化。实验结果表明,氧合血红蛋白和脱氧血红蛋白浓度变化的公式计算值与仪器测量值在液体模型实验中吻合较好,其相对误差平均值与标准偏差为14%±5%,因此,利用光源-探测器小距离散射光谱进行氧合血红蛋白和脱氧血红蛋白浓度变化分析的准确度高。在大鼠疼痛刺激实验中,前扣带回的氧合血红蛋白浓度在注射后的5 min内出现短期增大,随后单调减少。反之,脱氧血红蛋白浓度在注射后的5 min内出现短期减小,随后单调增大。总血红蛋白浓度在注射后没有显著性变化。前扣带回局部电信号功率在注射后逐渐增大,约20 min到峰值,之后保持在峰值附近,与注射前有显著性差异,可以确认氧合血红蛋白和脱氧血红蛋白浓度的变化是与前扣带回功能激活高度相关的。由此可见,基于光源-探测器小距离探头的散射光谱系统可以有效地用于标记与疼痛处理相关的核团的功能激活和分析核团的神经-血管耦合机制,为利用小动物模型增进对疼痛相关的脑血管功能的了解和疼痛测量提供了一种有效的新方式。A new method of pain measurement using scattering spectrum with a small source-detector separation is proposed.First,the relationship between the change of oxy-hemoglobin and deoxy-hemoglobin concentrations and the change of scattering spectrum intensity sampled through an optical probe with a small source-detector separation is established based on the modified Beer-Lambert’s law.The formula calculating results are compared with those measured by tissue oximeter in experiments using Intralipid mixed with blood.Secondly,the formalin solution was injected to seven rats’hind paws to create pain stimulation.The spectrum system with a small source-detector separation was used to measure the scattering spectrum of the anterior cingulate cortex(ACC)of rats before,and after injection,meanwhile,the local field potential of ACC before and after injection were acquired by the bioelectrical signal acquisition system.Finally,the change of oxy-hemoglobin,deoxy-hemoglobin and total hemoglobin concentration were calculated.The power spectrum intensity ofβwave(13~30 Hz)was obtained every 10 seconds,respectively.The changes in these parameters before and after injection were analyzed.The results showed that the values of the oxy-hemoglobin and deoxy-hemoglobin concentration change calculated by the formula are in good agreement with those measured by the Oximeter in the liquid model experiment,in which the average relative error with the standard deviation is 14%±5%.Therefore,the analysis of the change of oxy-hemoglobin and deoxy-hemoglobin concentration by the spectrum system with a small source-detector separation could have high accuracy.In the pain stimulation experiments of rats,the oxy-hemoglobin concentration of ACC increased rapidly for a short time within 5 minutes after the injection of formalin and then decreased monotonously.Meanwhile,the deoxy-hemoglobin concentration decreased in a short time within 5 minutes after injection and then increased monotonously.There was no significant change in total hemoglobin co

关 键 词：散射光谱 疼痛 血流动力学参数 光源-探测器小距离 

分 类 号：O433.4[机械工程—光学工程]