改良Fujita法制备兔心肌梗死模型  

Rabbit model of myocardial infarction prepared by modified Fujita method

作　　者：孙阿林 潘斌[2] 王健[3] SUN Alin;PAN Bin;WANG Jian(Intensive Care Unit,Weifang Peoples Hospital,Weifang 261041,China;Laboratory of Weifang Medical University;Department of Cardiology,Weifang People's Hospital)

机构地区：[1]潍坊市人民医院重症医学科,山东潍坊261041 [2]潍坊医学院实验室 [3]潍坊市人民医院心内科

出　　处：《潍坊医学院学报》2020年第4期309-311,F0004,共4页Acta Academiae Medicinae Weifang

基　　金：潍坊市卫计委项目(项目编号:2016wsjs103)。

摘　　要：目的改良Fujita法制备兔心肌梗死模型。方法选取新西兰大白兔共38只,随机分为假手术组9只,模型组29只。模型组通过改良Fujita法小切口(1.5~2.0cm)开胸,保证兔自主呼吸,不使用气管插管和呼吸机,双线结扎冠状动脉前降支并观察心肌组织颜色改变,记录十二导联心电图;术后4周心肌梗死区取材行HE染色,观察病理变化。假手术组开胸后,只穿线而不解扎冠状动脉。结果模型组新西兰兔结扎前降支后心肌组织变苍白,心电图记录在30min至数小时后显示急性广泛前壁心肌梗死图形改变,提示模型制备成功;模型组梗死心肌组织细胞变形、肿胀、破裂、坏死,细胞排列杂乱,周围炎症细胞浸润。假手术组心肌组织细胞排列规则,未见明显病理改变,无炎症细胞浸润;模型组制模成功率(82.76%)低于假手术组(88.89%),但差异无显著性(χ^2=0.016,P>0.05)。结论通过改良Fujita法小切口(1.5~2.0cm)开胸结扎冠状动脉前降支,结合心电图、组织学等改变,可成功制备兔心肌梗死模型,且成功率较高。Objective To prepare the rabbit model of myocardial infarction by the improved Fujita method.Methods Thirty-eighty New Zealand white rabbit were randomly divided into control group(9 cases) and model group(29 cases).In the model group,thoracotomy was performed through a modified Fujita method small incision(1.5~2.0 cm) to ensure that rabbits could breathe spontaneously,without using tracheal intubation and ventilator,double ligating the anterior descending coronary artery and observing the color change of myocardial tissue,recording 12-lead ECG;Four weeks after operation,the area of myocardial infarction was taken for HE staining and pathological changes were observed.And therabbits in control group were only thread puzzled coronary artery after thoracotomy.Results The myocardial tissue of the New Zealand rabbits before the ligation in the model group became pale,and the ECG recording showed acute extensive anterior myocardial infarction after 30 min,indicating that the model was successfully prepared;the infarcted myocardial cells in the model group were deformed,swollen,ruptured,and necrotic.In the model group,the infarcted myocardial cells were deformed,swollen,ruptured,necrotic,the cells were arranged disorderly,and the surrounding inflammatory cells infiltrated.The myocardial tissue cells in control group were regularly arranged without obvious pathological changes and no inflammatory cell infiltration.The success rate of the model group(82.76%) was lower than the control group(88.89%),but the difference was not significant(χ^2=0.016,P>0.05).Conclusion A modified Fujita method with a small incision(1.5~2.0 cm) can be used to ligate the anterior descending coronary artery,combined with ECG,histological changes,the rabbit myocardial infarction model could be successfully prepared with a high success rate.

关 键 词：心肌梗死 新西兰兔 模型 心电图 病理 

分 类 号：R542.22[医药卫生—心血管疾病]